Treffer: Development and validation of a direct homologous quantitative sandwich ELISA for fathead minnow (Pimephales promelas) vitellogenin

Title:
Development and validation of a direct homologous quantitative sandwich ELISA for fathead minnow (Pimephales promelas) vitellogenin
Authors:
Hans Kleivdal, Kevin J. Kroll, Grace H. Panter, Anders Goksøyr, Nancy D. Denslow, Charles R. Tyler, Ronny van Aerle, Janne K. Eidem, Thomas H. Hutchinson
Source:
Aquatic toxicology (Amsterdam, Netherlands). 78(2)
Publication Year:
2006
Subject Terms:
medicine.medical_specialty, Time Factors, Health, Toxicology and Mutagenesis, Population, Cyprinidae, Enzyme-Linked Immunosorbent Assay, Aquatic Science, Biology, Sensitivity and Specificity, Andrology, chemistry.chemical_compound, Common carp, Vitellogenin, Vitellogenins, biology.animal, Internal medicine, medicine, Animals, education, Chronic toxicity, education.field_of_study, Temperature, Antibodies, Monoclonal, Reproducibility of Results, Estrogens, Environmental Exposure, Minnow, Nonylphenol, Endocrinology, chemistry, Endocrine disruptor, biology.protein, Pimephales promelas
ISSN:
0166-445X
Access URL:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bac06823c3f01bcea828fb9f173a1872
https://pubmed.ncbi.nlm.nih.gov/16644026
Rights:
CLOSED
Accession Number:
edsair.doi.dedup.....bac06823c3f01bcea828fb9f173a1872
Database:
OpenAIRE

Weitere Informationen

Vitellogenin (Vtg) is an established and sensitive endpoint for analysis of exposure to (anti-)oestrogens and their mimics in fish [Sumpter, J.P., 1995. Feminized responses in fish to environmental estrogens. Toxicol. Lett. 82, 737–742; Arukwe, A., Goksoyr, A., 2003. Eggshell and egg yolk proteins in fish: hepatic proteins for the next generation: oogenetic, population, and evolutionary implications of endocrine disruption. Comp. Hepatol. 2, 4. http://www.comparative-hepatology.com]. In some instances, links have been drawn between high level induction of Vtg and adverse health effects in fish [Herman, R.L., Kincaide, H.L., 1988. Pathological effects of orally administered estradiol to rainbow trout. Aquaculture 72, 165–172; Schwaiger, J., Spieser, O.H., Bauer, C., Ferling, H., Mallow, U., Kalbfus, W., Negele, R.D., 2000. Chronic toxicity of nonylphenol and ethinyloestraiol: haematological and histopathological effects in juvenile common carp (Cyprinus carpio). Aquat. Toxicol. 51, 69–78]. The widespread use of Vtg as a biomarker has led to the development of a variety of assays to quantitatively measure Vtg concentrations in tissue samples from fish, and hence a need for a standardization of the performance criteria and validation of such assays [Goksoyr, A., Eidem, J.K., Kristiansen, S.I., Nilsen, B.M., 2003. On the need for a standardized set-up for validation studies of fish vitellogenin assays as an endpoint in endocrine disruptor testing and screening—a proposal. http://www.biosense.com/Docs/GoksoyrEtal2003.pdf]. One of the most popular test fish species for assessing chemical effects is the fathead minnow (Pimephales promelas), which is now used widely for studies into endocrine disruption [Panter, G.H., Hutchinson, T.H., Lange, R., Lye, C.M., Sumpter, J.P., Zerulla, M., Tyler, C.R., 2002. Utility of a juvenile fathead minnow screening assay for detecting (anti)estrogenic substances. Environ. Toxicol. Chem. 21, 319–326; Hutchinson, T.H., Yokota, H., Hagino, S., Ozato, K., 2003. Development of fish tests for endocrine disruptors. Pure Appl. Chem. 75, 2343–2353]. This paper describes the development and validation of a new, homologous enzyme-linked immunosorbent assay (ELISA) for quantification of Vtg in this fish species.