*Result*: One Health Insights into Enterococcus: Antimicrobial Resistance and Virulence in Companion Animals and Their Tutors.
Title:
One Health Insights into Enterococcus: Antimicrobial Resistance and Virulence in Companion Animals and Their Tutors.
Authors:
Monteiro Marques J; Centre for Interdisciplinary Research in Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal.; Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), 1300-477 Lisbon, Portugal.; National Institute of Agricultural and Veterinary Research, IP (INIAV), 2780-157 Oeiras, Portugal., Pita B; NOVA School of Science and Technology (NOVA FCT), NOVA University Lisbon, 2829-516 Caparica, Portugal., Pinto D; Centre for Interdisciplinary Research in Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal.; Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), 1300-477 Lisbon, Portugal., Barreto-Crespo MT; iBET-Instituto de Biologia Experimental e Tecnológica, 2780-157 Oeiras, Portugal.; Instituto de Tecnologia Química e Biológica António Xavier (ITQB), 2780-157 Oeiras, Portugal., Mato R; UCIBIO-Applied Molecular Biosciences Unit, Department of Chemistry/Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal.; Associate Laboratory i4HB-Institute for Health and Bioeconomy, NOVA School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, Portugal., Semedo-Lemsaddek T; Centre for Interdisciplinary Research in Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal.; Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), 1300-477 Lisbon, Portugal.; Biosystems & Integrative Sciences Institute (BioISI), Faculty of Sciences, University of Lisbon, 1749-016 Lisbon, Portugal.
Source:
International journal of molecular sciences [Int J Mol Sci] 2026 Jan 08; Vol. 27 (2). Date of Electronic Publication: 2026 Jan 08.
Publication Type:
Journal Article
Language:
English
Journal Info:
Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
Imprint Name(s):
Original Publication: Basel, Switzerland : MDPI, [2000-
MeSH Terms:
Enterococcus*/drug effects , Enterococcus*/pathogenicity , Enterococcus*/genetics , Enterococcus*/isolation & purification , Pets*/microbiology , Gram-Positive Bacterial Infections*/microbiology , Gram-Positive Bacterial Infections*/veterinary , Drug Resistance, Bacterial* , One Health*, Anti-Bacterial Agents/pharmacology ; Feces/microbiology ; Enterococcus faecalis/pathogenicity ; Enterococcus faecalis/drug effects ; Animals ; Humans ; Virulence ; Microbial Sensitivity Tests ; Portugal
References:
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Animals (Basel). 2021 Aug 09;11(8):. (PMID: 34438804)
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New Microbiol. 2015 Jul;38(3):369-78. (PMID: 26147147)
Gels. 2022 Nov 22;8(12):. (PMID: 36547284)
J Appl Microbiol. 2021 Oct;131(4):2061-2071. (PMID: 33725426)
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Microb Drug Resist. 2014 Oct;20(5):501-7. (PMID: 24617521)
J Vet Med Sci. 2023 Mar 28;85(3):371-378. (PMID: 36724969)
J Clin Microbiol. 2003 Jun;41(6):2569-76. (PMID: 12791882)
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Int J Food Microbiol. 2008 Mar 31;123(1-2):38-49. (PMID: 18180067)
Vet Rec. 2015 Jun 27;176(26):674. (PMID: 26078332)
Diagn Microbiol Infect Dis. 2000 Jun;37(2):127-37. (PMID: 10863107)
Front Microbiol. 2020 Jun 25;11:1266. (PMID: 32670224)
Front Cell Infect Microbiol. 2020 Feb 28;10:73. (PMID: 32185142)
BMC Microbiol. 2007 Dec 11;7:112. (PMID: 18070365)
Antibiotics (Basel). 2021 Jan 31;10(2):. (PMID: 33572528)
J Appl Microbiol. 2009 Oct;107(4):1269-78. (PMID: 19486402)
Appl Environ Microbiol. 2001 Apr;67(4):1628-35. (PMID: 11282615)
Lett Appl Microbiol. 2006 Jun;42(6):553-9. (PMID: 16706891)
Front Vet Sci. 2024 Oct 21;11:1458069. (PMID: 39497740)
Antibiotics (Basel). 2022 May 03;11(5):. (PMID: 35625259)
Microb Drug Resist. 2018 Jun;24(5):590-606. (PMID: 29058560)
J Clin Microbiol. 2014 May;52(5):1582-9. (PMID: 24599985)
Res Vet Sci. 2024 May;171:105220. (PMID: 38484448)
Folia Microbiol (Praha). 2017 Nov;62(6):491-498. (PMID: 28316009)
Front Microbiol. 2023 Jan 05;13:1025242. (PMID: 36687655)
J Microbiol Methods. 2000 Oct;42(2):139-47. (PMID: 11018270)
Microbiol Mol Biol Rev. 2001 Jun;65(2):232-60 ; second page, table of contents. (PMID: 11381101)
Proc Natl Acad Sci U S A. 2012 Oct 16;109(42):16900-5. (PMID: 23027944)
Nat Commun. 2022 Jan 26;13(1):509. (PMID: 35082278)
Vector Borne Zoonotic Dis. 2024 Sep;24(9):600-606. (PMID: 38800841)
Future Microbiol. 2024;19(16):1407-1418. (PMID: 39229839)
J Appl Microbiol. 2010 May;108(5):1563-75. (PMID: 19804536)
Food Microbiol. 2013 Dec;36(2):465-74. (PMID: 24010630)
Microbiol Spectr. 2018 Jul;6(4):. (PMID: 30051804)
Clin Microbiol Infect. 2012 Mar;18(3):268-81. (PMID: 21793988)
Front Pharmacol. 2025 Apr 07;16:1505674. (PMID: 40260375)
J Antimicrob Chemother. 2007 May;59(5):1017-20. (PMID: 17369277)
Lancet Microbe. 2022 Feb;3(2):e133-e141. (PMID: 35146465)
Ann Clin Microbiol Antimicrob. 2024 Aug 24;23(1):80. (PMID: 39182092)
J Antimicrob Chemother. 2018 Mar 1;73(3):607-614. (PMID: 29294027)
Can J Microbiol. 2024 Aug 1;70(8):303-335. (PMID: 38696839)
J Am Vet Med Assoc. 2012 Feb 15;240(4):437-45. (PMID: 22309016)
Microb Drug Resist. 2020 Oct;26(10):1174-1185. (PMID: 32354251)
Exp Mol Med. 2021 Mar;53(3):301-309. (PMID: 33642573)
Antibiotics (Basel). 2020 Mar 31;9(4):. (PMID: 32244399)
J Clin Microbiol. 2006 Apr;44(4):1567-70. (PMID: 16597896)
Vet Sci. 2022 Apr 24;9(5):. (PMID: 35622736)
Appl Environ Microbiol. 2005 Sep;71(9):5383-90. (PMID: 16151128)
Comp Immunol Microbiol Infect Dis. 2008 Jan;31(1):1-9. (PMID: 17881054)
Animals (Basel). 2021 Sep 29;11(10):. (PMID: 34679866)
BMC Vet Res. 2025 Feb 25;21(1):99. (PMID: 39994632)
J Vet Med B Infect Dis Vet Public Health. 2002 Aug;49(6):278-80. (PMID: 12241027)
J Clin Microbiol. 2004 Aug;42(8):3558-65. (PMID: 15297497)
Appl Environ Microbiol. 2025 Jan 31;91(1):e0169924. (PMID: 39699199)
Microorganisms. 2025 Jul 03;13(7):. (PMID: 40732073)
3 Biotech. 2013 Apr;3(2):97-107. (PMID: 28324565)
Water Res. 2006 May;40(8):1735-40. (PMID: 16603222)
Vet Microbiol. 2013 May 3;163(3-4):378-82. (PMID: 23375652)
Animals (Basel). 2021 Aug 09;11(8):. (PMID: 34438804)
Acta Microbiol Immunol Hung. 2017 Sep 1;64(3):301-312. (PMID: 27976593)
Int J Antimicrob Agents. 2007 Jul;30(1):98-100. (PMID: 17509838)
Appl Environ Microbiol. 2001 Jan;67(1):22-32. (PMID: 11133424)
Acta Vet Hung. 2017 Jun;65(2):173-184. (PMID: 28605961)
Syst Appl Microbiol. 2003 Mar;26(1):13-22. (PMID: 12747405)
Antimicrob Agents Chemother. 2001 May;45(5):1480-6. (PMID: 11302814)
New Microbiol. 2015 Jul;38(3):369-78. (PMID: 26147147)
Gels. 2022 Nov 22;8(12):. (PMID: 36547284)
J Appl Microbiol. 2021 Oct;131(4):2061-2071. (PMID: 33725426)
Appl Environ Microbiol. 2006 Jun;72(6):4028-35. (PMID: 16751512)
Antimicrob Agents Chemother. 1975 Dec;8(6):713-20. (PMID: 1211925)
J Clin Microbiol. 1999 Nov;37(11):3497-503. (PMID: 10523541)
J Vet Med Sci. 2014 Oct;76(10):1399-402. (PMID: 24976587)
Microb Drug Resist. 2014 Oct;20(5):501-7. (PMID: 24617521)
J Vet Med Sci. 2023 Mar 28;85(3):371-378. (PMID: 36724969)
J Clin Microbiol. 2003 Jun;41(6):2569-76. (PMID: 12791882)
J Clin Microbiol. 1998 Feb;36(2):592-4. (PMID: 9466786)
J Clin Microbiol. 2001 Jun;39(6):2367-8. (PMID: 11414244)
Int J Food Microbiol. 2008 Mar 31;123(1-2):38-49. (PMID: 18180067)
Vet Rec. 2015 Jun 27;176(26):674. (PMID: 26078332)
Diagn Microbiol Infect Dis. 2000 Jun;37(2):127-37. (PMID: 10863107)
Front Microbiol. 2020 Jun 25;11:1266. (PMID: 32670224)
Front Cell Infect Microbiol. 2020 Feb 28;10:73. (PMID: 32185142)
BMC Microbiol. 2007 Dec 11;7:112. (PMID: 18070365)
Antibiotics (Basel). 2021 Jan 31;10(2):. (PMID: 33572528)
J Appl Microbiol. 2009 Oct;107(4):1269-78. (PMID: 19486402)
Appl Environ Microbiol. 2001 Apr;67(4):1628-35. (PMID: 11282615)
Lett Appl Microbiol. 2006 Jun;42(6):553-9. (PMID: 16706891)
Front Vet Sci. 2024 Oct 21;11:1458069. (PMID: 39497740)
Antibiotics (Basel). 2022 May 03;11(5):. (PMID: 35625259)
Microb Drug Resist. 2018 Jun;24(5):590-606. (PMID: 29058560)
J Clin Microbiol. 2014 May;52(5):1582-9. (PMID: 24599985)
Res Vet Sci. 2024 May;171:105220. (PMID: 38484448)
Folia Microbiol (Praha). 2017 Nov;62(6):491-498. (PMID: 28316009)
Front Microbiol. 2023 Jan 05;13:1025242. (PMID: 36687655)
J Microbiol Methods. 2000 Oct;42(2):139-47. (PMID: 11018270)
Microbiol Mol Biol Rev. 2001 Jun;65(2):232-60 ; second page, table of contents. (PMID: 11381101)
Proc Natl Acad Sci U S A. 2012 Oct 16;109(42):16900-5. (PMID: 23027944)
Nat Commun. 2022 Jan 26;13(1):509. (PMID: 35082278)
Vector Borne Zoonotic Dis. 2024 Sep;24(9):600-606. (PMID: 38800841)
Future Microbiol. 2024;19(16):1407-1418. (PMID: 39229839)
Grant Information:
2024.03583.BDANA Fundação para a Ciência e Tecnologia; UIDB/00276/2020 Fundação para a Ciência e Tecnologia; LA/P/0059/2020 Fundação para a Ciência e Tecnologia; UID/04462: iNOVA4Health Fundação para a Ciência e Tecnologia; LA/P/0087/2020 Fundação para a Ciência e Tecnologia
Contributed Indexing:
Keywords: Enterococcus; One Health; antimicrobial resistance; companion animals; healthy humans; virulence
Substance Nomenclature:
0 (Anti-Bacterial Agents)
Entry Date(s):
Date Created: 20260128 Date Completed: 20260128 Latest Revision: 20260131
Update Code:
20260131
PubMed Central ID:
PMC12841424
DOI:
10.3390/ijms27020654
PMID:
41596304
Database:
MEDLINE
*Further Information*
*Enterococcus spp. are opportunistic pathogens and commensals in humans and animals and are widely used as indicators of bacterial exchange, providing insights into antimicrobial resistance (AMR) and virulence dissemination within the One Health continuum. Enterococcus from healthy companion animals and their tutors were characterized to compare AMR profiles and virulence traits between hosts and within households in Lisbon, Portugal. Fecal samples (n = 45) were collected from 17 animals and 11 tutors. Enterococci were recovered from selective media, subjected to random amplified polymorphic DNA PCR (RAPD-PCR) and species identification, tested for antimicrobial susceptibility, and screened for virulence traits. Among animal isolates, 61% were Enterococcus faecalis, 29% E. faecium, and 10% E. hirae, whereas human enterococci comprised 52% E. faecalis, 35% E. faecium, 8% E. hirae, and 4% other species. Erythromycin resistance was identical in both groups (29%; Chi-squared test, p = 0.99). Ampicillin resistance was detected in all animal samples but was absent in human samples, whereas tetracycline and rifampicin resistance showed moderate host-specific patterns. Hemolytic activity was detected in 16% of animal and 31% of human isolates, all cylA-positive. Significant associations were observed between host origin and resistance to ampicillin and rifampicin, and between species and resistance to erythromycin and tetracycline. These findings suggest that companion animals can harbor, and potentially disseminate, AMR and virulence traits, reinforcing the need for One Health surveillance.*