*Result*: Exploring the Phytochemical Profile and Biological Activities, Supported by Molecular Docking and Dynamics Simulation Studies of Lysimachia tyrrhenia U.Manns & Anderb.
Title:
Exploring the Phytochemical Profile and Biological Activities, Supported by Molecular Docking and Dynamics Simulation Studies of Lysimachia tyrrhenia U.Manns & Anderb.
Authors:
Djeblia L; Département de Chimie, Laboratoire d'Obtention Des Substances Thérapeutiques (LOST), Campus Chaabet-Ersas, Université Des Frères Mentouri-Constantine 1, Constantine, Algeria.; Laboratoire de Biotechnologie des Molécules Bioactives Et Physiopathologie Cellulaire Mustapha Ben Boulaid, Département de Microbiologie et Biochimie, Université Batna 2, Batna, Algeria.; CNRS, ICMR UMR 7312, Université De Reims Champagne Ardenne, Reims, France., Boutaghane N; Département de Chimie, Laboratoire d'Obtention Des Substances Thérapeutiques (LOST), Campus Chaabet-Ersas, Université Des Frères Mentouri-Constantine 1, Constantine, Algeria., Bendamene S; Département de Chimie, Laboratoire d'Obtention Des Substances Thérapeutiques (LOST), Campus Chaabet-Ersas, Université Des Frères Mentouri-Constantine 1, Constantine, Algeria.; Université 20 Aout 1955 Skikda, Skikda, Algeria., Kenza B; Département de Chimie, Laboratoire d'Obtention Des Substances Thérapeutiques (LOST), Campus Chaabet-Ersas, Université Des Frères Mentouri-Constantine 1, Constantine, Algeria., Voutquenne-Nazabadioko L; CNRS, ICMR UMR 7312, Université De Reims Champagne Ardenne, Reims, France., Boulebd H; Laboratory of Synthesis of Molecules with Biological Interest, Department of Chemistry, Faculty of Exact Sciences, University Frères Mentouri Constantine 1, Constantine, Algeria., May A; Department of Chemistry, University Mentouri-Constantine 1, Constantine, Algeria., Kumar S; Faculty of Health and Life Sciences, Management and Science University, Shah Alam, Malaysia., Kumar KM; Department of Bioinformatics, School of Life Science, Pondicherry University, (A Central University, Govt of India) Chinna Kalapet, Puducherry, India., Yilmaz MA; Department of Analytical Chemistry, Faculty of Pharmacy, Dicle University Science and Technology Research and Application Center (DUBTAM), Diyarbakır, Turkey., Kabouche Z; Département de Chimie, Laboratoire d'Obtention Des Substances Thérapeutiques (LOST), Campus Chaabet-Ersas, Université Des Frères Mentouri-Constantine 1, Constantine, Algeria., Hambaba L; Laboratoire de Biotechnologie des Molécules Bioactives Et Physiopathologie Cellulaire Mustapha Ben Boulaid, Département de Microbiologie et Biochimie, Université Batna 2, Batna, Algeria.
Source:
Chemistry & biodiversity [Chem Biodivers] 2026 Jan; Vol. 23 (1), pp. e03381.
Publication Type:
Journal Article
Language:
English
Journal Info:
Publisher: Verlag Helvetica Chimica Acta Country of Publication: Switzerland NLM ID: 101197449 Publication Model: Print Cited Medium: Internet ISSN: 1612-1880 (Electronic) Linking ISSN: 16121872 NLM ISO Abbreviation: Chem Biodivers Subsets: MEDLINE
Imprint Name(s):
Original Publication: Zürich, Switzerland : Hoboken, NJ : Verlag Helvetica Chimica Acta ; Distributed in the USA by Wiley, c2004-
MeSH Terms:
Cholinesterase Inhibitors*/chemistry , Cholinesterase Inhibitors*/pharmacology , Cholinesterase Inhibitors*/isolation & purification , Phytochemicals*/chemistry , Phytochemicals*/pharmacology , Phytochemicals*/isolation & purification , Antioxidants*/chemistry , Antioxidants*/pharmacology , Antioxidants*/isolation & purification , Primulaceae*/chemistry , Primulaceae*/metabolism , Plant Extracts*/chemistry , Plant Extracts*/pharmacology , Plant Extracts*/isolation & purification , Molecular Docking Simulation*, Acetylcholinesterase/metabolism ; Biphenyl Compounds/antagonists & inhibitors ; Picrates/antagonists & inhibitors ; Butyrylcholinesterase/metabolism ; Molecular Dynamics Simulation ; Tandem Mass Spectrometry ; Lysimachia
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Grant Information:
B00L01UN250120220003 DGRSDT of the Ministry of Higher Education and Scientific Research of Algeria for PRFU Project; Biotechnology Research Center (CRBt) (Constantine-Algeria); Conseil Regional Champagne Ardenne; Conseil General de la Marne, Ministry of Higher Education and Research (MESR) in France; PlANET CPER Project
Contributed Indexing:
Keywords: LC–ESI–MS/MS analysis; Lysimachia tyrrhenia; anticholinesterase activity; antioxidant activity; dynamics simulation; molecular docking; sun protective activity
Substance Nomenclature:
0 (Cholinesterase Inhibitors)
0 (Phytochemicals)
0 (Antioxidants)
EC 3.1.1.7 (Acetylcholinesterase)
0 (Plant Extracts)
0 (Biphenyl Compounds)
0 (Picrates)
EC 3.1.1.8 (Butyrylcholinesterase)
0 (Phytochemicals)
0 (Antioxidants)
EC 3.1.1.7 (Acetylcholinesterase)
0 (Plant Extracts)
0 (Biphenyl Compounds)
0 (Picrates)
EC 3.1.1.8 (Butyrylcholinesterase)
Entry Date(s):
Date Created: 20260114 Date Completed: 20260114 Latest Revision: 20260114
Update Code:
20260130
DOI:
10.1002/cbdv.202503381
PMID:
41532854
Database:
MEDLINE
*Further Information*
*The current research was conducted to explore for the first time the phytochemical composition, of the methanolic extract and the fractions of Lysimachia tyrrhenia U.Manns & Anderb. using a developed LC-ESI-MS/MS method. In addition, antioxidant, cholinesterase inhibitory, and photoprotective activities were evaluated to highlight the potential therapeutic relevance of this species. In vitro trials were undertaken to investigate the antioxidant activity of the L. tyrrhenia sampls based on 2-diphenyl-1-picrylhydrazyl (DPPH<sup>•</sup>), 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS<sup>•+</sup>), cupric reducing antioxidant capacity (CUPRAC), ferric ions (Fe<sup>3+</sup>) reducing assay (FRAP) and phenanthroline. The photoprotective potential of the L. tyrrhenia extracts against UV-induced damage was assessed through in vitro sun protection factor (SPF) measurements, while the neuroprotective effect was examined in vitro against acetylcholinesterase (AChE) enzyme. This study accurately estimated the chemical bonding between the identified molecules derived from LC-MS/MS and the AChE. The LC-MS/MS results revealed that rutin, epicatechin, hesperidin, are the abundant compounds in most of the samples. All studied samples demonstrated remarkable antioxidant potentials, among them the fraction I was the strongest, with IC<subscript>50</subscript> values of 11.71 ± 0.17 µg/mL (DPPH), 1.82 ± 0.05 µg/mL (ABTS), 4.69 ± 0.15 µg/mL (CUPRAC), and 1.00 ± 0.07 µg/mL (phenanthroline). It also exerted the considerable AChE inhibitory effect (IC<subscript>50</subscript> = 58.31 ± 0.18 µg/mL). Molecular docking studies have highlighted hesperidin as a promising AChE inhibitor, with a binding energy of -10.5 kcal/mol. Molecular dynamic simulations further support hesperidin's potential, showing stable interactions with AChE. Moreover, all fractions showed high photoprotective activity with the SPF value ranged from 34.58 to 35.19. These results highlight the therapeutic potential of L. tyrrhenia as a source of bioactive compounds for developing new approaches for managing cholinesterase-related disorders and oxidative stress.
(© 2026 Wiley‐VHCA AG.)*