• Integrated analysis of dengue...

*Result*: Integrated analysis of dengue transmission risk and insecticide resistance in Aedes aegypti from Vichada, Colombia.

Title:
Integrated analysis of dengue transmission risk and insecticide resistance in Aedes aegypti from Vichada, Colombia.
Authors:
Mejía-Jaramillo AM; Biology and Infectious Diseases Control Group, Universidad de Antioquia, Medellín, Colombia., Cantillo-Barraza O; Biology and Infectious Diseases Control Group, Universidad de Antioquia, Medellín, Colombia., Medina CS; Biology and Infectious Diseases Control Group, Universidad de Antioquia, Medellín, Colombia.; Entomology Unit, Public Health Laboratory, Department of Health of Vichada, Puerto Carreño, Colombia., Jaimes-Dueñez J; Animal Science Research Group, Faculty of Veterinary Medicine and Animal Husbandry, Universidad Cooperativa de Colombia, Bucaramanga, Colombia., Triana-Chávez O; Biology and Infectious Diseases Control Group, Universidad de Antioquia, Medellín, Colombia.
Source:
Narra J [Narra J] 2025 Nov; Vol. 5 (3), pp. e2795. Date of Electronic Publication: 2025 Nov 19.
Publication Type:
Journal Article
Language:
English
Journal Info:
Publisher: Narra Sains Indonesia Country of Publication: Indonesia NLM ID: 9918625888906676 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2807-2618 (Electronic) Linking ISSN: 28072618 NLM ISO Abbreviation: Narra J Subsets: MEDLINE
Imprint Name(s):
Original Publication: Banda Aceh, Indonesia : Narra Sains Indonesia, [2021]-
MeSH Terms:
Aedes*/virology , Aedes*/genetics , Aedes*/drug effects , Insecticide Resistance*/genetics , Dengue*/transmission , Dengue*/epidemiology , Dengue*/prevention & control , Mosquito Vectors*/virology , Mosquito Vectors*/genetics , Mosquito Vectors*/drug effects , Dengue Virus*/genetics, Colombia/epidemiology ; Insecticides/pharmacology ; Animals ; Humans
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Contributed Indexing:
Keywords: Aedes aegypti; Dengue; insecticide resistance; kdr mutations; vector surveillance
Molecular Sequence:
figshare 10.6084/m9.figshare.30644522
Substance Nomenclature:
0 (Insecticides)
Entry Date(s):
Date Created: 20260114 Date Completed: 20260114 Latest Revision: 20260225
Update Code:
20260225
PubMed Central ID:
PMC12793905
DOI:
10.52225/narra.v5i3.2795
PMID:
41531540
Database:
MEDLINE

*Further Information*

*Dengue is the primary arbovirus transmitted by Aedes aegypti mosquitoes. Effective management of dengue demands a multidisciplinary approach. The aim of this study was to conduct an integrated analysis of dengue transmission, focusing on its vector mosquitoes, to establish a baseline for dengue control and prevention in an endemic region of Colombia. The study was conducted from 2015 to 2018 across four municipalities in the Vichada department near the Venezuelan border. Five complementary approaches were employed: (1) determining the natural infection rate and circulation of various dengue virus serotypes in mosquitoes; (2) evaluating the insecticide susceptibility status and examining mosquito genotypes for three knockdown (kdr) mutations linked to insecticide resistance; (3) performing a phylogenetic analysis to identify the lineage of Ae. aegypti; (4) creating risk maps for dengue transmission based on predictive models in two municipalities in the department; and (5) empowering the community. Molecular analysis using RT-PCR indicated dengue virus infections in all municipalities of the Vichada department. Eleven positive pools for serotypes DENV-1, DENV-2, DENV-3, and DENV-4 were detected. The highest minimum infection rate (MIR) was found in Cumaribo, followed by La Primavera, which showed the greatest diversity of dengue virus serotypes. Mosquitoes from all three populations showed susceptibility to malathion and lambda-cyhalothrin pyrethroid, except for mosquitoes from Puerto Carreño, which presented moderate resistance to lambda-cyhalothrin (resistance ratio of 8). Importantly, all mosquitoes had F1534C mutation, while the V1016I and V419L mutations were found at lower frequencies. Risk classification maps for Puerto Carreño and La Primavera showed neighborhoods with high risk, indicating potential hotspots for intervention and vector control. This study established a necessary baseline for the ongoing monitoring and improvement of the early warning system for all municipalities in the Vichada department. The integrative approach employed in this study highlights the importance of incorporating these methodologies into dengue epidemiological surveillance in endemic regions.
(© 2025 The Author(s).)*

*All the authors declare that there are no conflicts of interest.*