• Transcriptome analysis reveals...

*Result*: Transcriptome analysis reveals the response mechanism of largemouth bass (Micropterus salmoides) brain to MSRV infection.

Title:
Transcriptome analysis reveals the response mechanism of largemouth bass (Micropterus salmoides) brain to MSRV infection.
Authors:
Guo Q; Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, PR China., Wang P; Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, PR China., Lou B; Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, PR China; State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, PR China., Shen W; Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, PR China., Han M; Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, PR China., Guan W; Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, PR China., Lyu S; Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, PR China. Electronic address: lvsunjian@163.com., Liu L; Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, PR China. Electronic address: liuli6655@hotmail.com.
Source:
Fish & shellfish immunology [Fish Shellfish Immunol] 2026 Mar; Vol. 170, pp. 111109. Date of Electronic Publication: 2026 Jan 08.
Publication Type:
Journal Article
Language:
English
Journal Info:
Publisher: Academic Press Country of Publication: England NLM ID: 9505220 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9947 (Electronic) Linking ISSN: 10504648 NLM ISO Abbreviation: Fish Shellfish Immunol Subsets: MEDLINE
Imprint Name(s):
Original Publication: London ; New York : Academic Press, c1991-
MeSH Terms:
Bass*/immunology , Bass*/genetics , Fish Diseases*/immunology , Fish Diseases*/virology , Fish Diseases*/genetics , Transcriptome*/immunology , Rhabdoviridae Infections*/veterinary , Rhabdoviridae Infections*/immunology , Rhabdoviridae Infections*/virology , Immunity, Innate*/genetics, Gene Expression Profiling/veterinary ; Brain/immunology ; Brain/virology ; Rhabdoviridae/physiology ; Fish Proteins/genetics ; Fish Proteins/immunology ; Animals
Contributed Indexing:
Keywords: Energy metabolism; Immune response; Largemouth bass; MSRV
Substance Nomenclature:
0 (Fish Proteins)
Entry Date(s):
Date Created: 20260110 Date Completed: 20260204 Latest Revision: 20260204
Update Code:
20260205
DOI:
10.1016/j.fsi.2026.111109
PMID:
41519215
Database:
MEDLINE

*Further Information*

*Micropterus salmoides is an economically significant freshwater fish that faces serious threats from various pathogens. Micropterus salmoides rhabdovirus (MSRV) is a pathogenic virus that primarily infects juvenile fish, leading to severe disease. Although some studies have reported the transcriptome sequencing of immune-related tissues in Micropterus salmoides after MSRV infection, studies focusing on the central nervous system remain scarce. In this study, largemouth bass juvenile fish were infected by intraperitoneal injection of MSRV. Severe external clinical sign, together with high virus load were detected in brain tissues after MSRV infection. Transcriptomic analysis showed that the expression level of 658 genes (297 up-regulated genes and 361 down-regulated) in brain tissues was significantly altered. First, KEGG analysis indicated that differentially expressed genes (DEGs) were positively enriched in the pathways related to immune signaling and cell death, such as JAK-STAT signaling pathway, cytokine-cytokine receptor interaction and apoptosis. Next, Most DEGs within the significant enrichment of "Citrate cycle", "Oxidative phosphorylation", "proteasome" and "ribosome" were downregulated in brain tissues infected with MSRV. Additionally, we found that MSRV infection reduces both the mitochondrial membrane potential and ATP production in largemouth bass brain tissues, thereby inhibiting viral replication. Altogether, this study revealed immune-related genes and pathways that defend against MSRV infection, as well as other cellular processes potentially involved in host antiviral responses or viral invasion. These findings are pending functional validation. All these results provided a reference for further in-depth study mechanisms of viral-host interactions in teleost fish.
(Copyright © 2026 Elsevier Ltd. All rights reserved.)*

*Conflict of interest statement We declare that we have no conflict of interest.*