*Result*: Comparative transcriptome analysis of developmental stages and characterization of core RNAi-related genes in the spittlebug Mahanarva fimbriolata.
Title:
Comparative transcriptome analysis of developmental stages and characterization of core RNAi-related genes in the spittlebug Mahanarva fimbriolata.
Authors:
Begnami IDS; Center for Plant Molecular Breeding (CeM²P), University of Campinas (UNICAMP), Campinas, SP, Brazil.; Center for Molecular Biology and Genetic Engineering, Biology Institute, University of Campinas (UNICAMP), SP, Campinas, Brazil., Gonçalves GFF; Department of Genetic and Evolution, University of de São Carlos, SP, São Carlos, Brazil.; Embrapa Pecuária Sudeste, SP, São Carlos, Brazil., Pimenta RJG; Center for Molecular Biology and Genetic Engineering, Biology Institute, University of Campinas (UNICAMP), SP, Campinas, Brazil.; Forestry Development Department, Teagasc, IE-L, Carlow, Ireland., Moraes ADCL; Center for Plant Molecular Breeding (CeM²P), University of Campinas (UNICAMP), Campinas, SP, Brazil.; Department of Plant Biology, Biology Institute, University of Campinas (UNICAMP), SP, Campinas, Brazil., Malagó Júnior W; Center for Plant Molecular Breeding (CeM²P), University of Campinas (UNICAMP), Campinas, SP, Brazil.; Embrapa Pecuária Sudeste, SP, São Carlos, Brazil., Gusmão MR; Center for Plant Molecular Breeding (CeM²P), University of Campinas (UNICAMP), Campinas, SP, Brazil.; Embrapa Pecuária Sudeste, SP, São Carlos, Brazil., de Souza AP; Center for Plant Molecular Breeding (CeM²P), University of Campinas (UNICAMP), Campinas, SP, Brazil.; Center for Molecular Biology and Genetic Engineering, Biology Institute, University of Campinas (UNICAMP), SP, Campinas, Brazil.; Department of Plant Biology, Biology Institute, University of Campinas (UNICAMP), SP, Campinas, Brazil., Vigna BBZ; Center for Plant Molecular Breeding (CeM²P), University of Campinas (UNICAMP), Campinas, SP, Brazil. bianca.vigna@embrapa.br.; Embrapa Pecuária Sudeste, SP, São Carlos, Brazil. bianca.vigna@embrapa.br.
Source:
BMC genomics [BMC Genomics] 2026 Jan 05; Vol. 27 (1), pp. 125. Date of Electronic Publication: 2026 Jan 05.
Publication Type:
Journal Article; Comparative Study
Language:
English
Journal Info:
Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2164 (Electronic) Linking ISSN: 14712164 NLM ISO Abbreviation: BMC Genomics Subsets: MEDLINE
Imprint Name(s):
Original Publication: London : BioMed Central, [2000-
MeSH Terms:
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Insect Sci. 2023 Oct;30(5):1309-1324. (PMID: 36763354)
Insect Mol Biol. 2016 Jun;25(3):315-23. (PMID: 26959524)
Nucleic Acids Res. 2011 Nov 1;39(20):8703-11. (PMID: 21764773)
Insects. 2020 Aug 20;11(9):. (PMID: 32825516)
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Curr Opin Insect Sci. 2015 Oct;11:39-46. (PMID: 28285758)
Front Genet. 2022 Jun 23;13:880000. (PMID: 35812742)
Nat Methods. 2012 Mar 04;9(4):357-9. (PMID: 22388286)
Sci Rep. 2018 Feb 1;8(1):2061. (PMID: 29391456)
Insect Sci. 2022 Apr;29(2):344-362. (PMID: 34388292)
J Econ Entomol. 2016 Aug;109(4):1914-21. (PMID: 27329631)
Arch Insect Biochem Physiol. 2025 Jun;119(2):e70069. (PMID: 40497425)
Front Plant Sci. 2021 Jan 28;11:526455. (PMID: 33584737)
Annu Rev Entomol. 2018 Jan 7;63:489-511. (PMID: 29058980)
Front Plant Sci. 2022 Apr 01;13:804839. (PMID: 35432425)
Mol Biol Rep. 2021 May;48(5):4383-4396. (PMID: 34091816)
Pest Manag Sci. 2021 May;77(5):2188-2196. (PMID: 33099867)
Nature. 2003 Sep 25;425(6956):415-9. (PMID: 14508493)
PLoS Negl Trop Dis. 2023 Jun 26;17(6):e0011411. (PMID: 37363930)
Front Cell Dev Biol. 2023 Jul 27;11:1190258. (PMID: 37576597)
Sci Rep. 2024 Oct 18;14(1):24451. (PMID: 39424855)
Mol Biol Evol. 2021 Jun 25;38(7):3022-3027. (PMID: 33892491)
Cell Rep. 2017 Jan 17;18(3):762-776. (PMID: 28099853)
Pest Manag Sci. 2019 Jan;75(1):18-28. (PMID: 29931761)
J Econ Entomol. 2020 Feb 8;113(1):115-125. (PMID: 31560771)
Methods. 2001 Dec;25(4):402-8. (PMID: 11846609)
BMC Bioinformatics. 2008 Dec 29;9:559. (PMID: 19114008)
PLoS One. 2023 Jan 3;18(1):e0279983. (PMID: 36595511)
Nat Rev Genet. 2011 Sep 07;12(10):671-82. (PMID: 21897427)
Bioinformatics. 2017 Sep 15;33(18):2938-2940. (PMID: 28645171)
BMC Genomics. 2019 Dec 9;20(1):954. (PMID: 31818245)
Arch Insect Biochem Physiol. 2019 Feb;100(2):e21522. (PMID: 30484903)
Nucleic Acids Res. 2000 Jan 1;28(1):27-30. (PMID: 10592173)
Bull Entomol Res. 2004 Jun;94(3):189-200. (PMID: 15191620)
Nat Methods. 2017 Apr;14(4):417-419. (PMID: 28263959)
Genomics. 2020 Mar;112(2):1651-1659. (PMID: 31626898)
Insect Biochem Mol Biol. 2018 Nov;102:75-83. (PMID: 30287269)
GigaByte. 2022 Feb 16;2022:gigabyte41. (PMID: 36824510)
Nucleic Acids Res. 1994 Nov 11;22(22):4673-80. (PMID: 7984417)
Wiley Interdiscip Rev Dev Biol. 2015 Jul-Aug;4(4):313-34. (PMID: 25758712)
Bioinformatics. 2012 Dec 1;28(23):3150-2. (PMID: 23060610)
Bioengineered. 2015;6(1):8-19. (PMID: 25424593)
PLoS One. 2015 Jun 19;10(6):e0130328. (PMID: 26091106)
Sci Rep. 2020 Jul 1;10(1):10758. (PMID: 32612122)
Cell. 2009 Feb 20;136(4):642-55. (PMID: 19239886)
Insect Mol Biol. 2023 Oct;32(5):528-543. (PMID: 37162032)
Development. 2009 Nov;136(21):3597-606. (PMID: 19793892)
Front Insect Sci. 2022 Jan 05;1:818037. (PMID: 38468882)
Curr Biol. 2019 Dec 2;29(23):R1252-R1268. (PMID: 31794762)
Pest Manag Sci. 2017 Jan;73(1):206-216. (PMID: 27060284)
Bioinformatics. 2012 Dec 15;28(24):3211-7. (PMID: 23071270)
Insect Mol Biol. 2010 Dec;19(6):777-86. (PMID: 20726907)
Arch Insect Biochem Physiol. 2020 Aug;104(4):e21680. (PMID: 32346914)
Nat Biotechnol. 2011 May 15;29(7):644-52. (PMID: 21572440)
Sci Rep. 2018 Mar 16;8(1):4729. (PMID: 29549300)
Annu Rev Entomol. 2020 Jan 7;65:293-311. (PMID: 31610134)
Int J Biol Macromol. 2021 Feb 1;169:216-227. (PMID: 33340629)
FEBS Lett. 2005 Oct 31;579(26):5822-9. (PMID: 16214139)
Chromosome Res. 2023 Jan 27;31(1):5. (PMID: 36705735)
Sci Rep. 2020 Mar 17;10(1):4856. (PMID: 32184426)
Curr Opin Insect Sci. 2021 Feb;43:29-38. (PMID: 33075581)
Bioinformatics. 2014 Aug 1;30(15):2114-20. (PMID: 24695404)
BMC Genomics. 2018 Sep 21;19(1):693. (PMID: 30241467)
Nucleic Acids Res. 2013 Jul;41(12):e121. (PMID: 23598997)
Mol Biotechnol. 2022 Oct;64(10):1152-1163. (PMID: 35460447)
Insects. 2020 Sep 06;11(9):. (PMID: 32899929)
Sci Rep. 2020 Feb 20;10(1):3091. (PMID: 32080221)
Fly (Austin). 2017 Jul 3;11(3):218-223. (PMID: 28121487)
BMC Genomics. 2022 Sep 8;23(1):636. (PMID: 36076177)
Curr Opin Insect Sci. 2025 Feb;67:101289. (PMID: 39490982)
Nat Genet. 2000 May;25(1):25-9. (PMID: 10802651)
Eukaryot Cell. 2008 Mar;7(3):429-34. (PMID: 17951525)
Front Mol Biosci. 2023 Mar 06;10:1073721. (PMID: 36950526)
PLoS One. 2015 Jan 15;10(1):e0115336. (PMID: 25590333)
Trends Biotechnol. 2008 Jul;26(7):393-400. (PMID: 18501983)
PLoS One. 2023 Mar 1;18(3):e0277815. (PMID: 36857331)
Insect Sci. 2018 Jun;25(3):356-367. (PMID: 28058810)
Arch Insect Biochem Physiol. 2013 Dec;84(4):194-208. (PMID: 24132808)
Int J Mol Sci. 2024 Feb 07;25(4):. (PMID: 38396690)
Bioinformatics. 2015 Oct 1;31(19):3210-2. (PMID: 26059717)
Neotrop Entomol. 2014 Feb;43(1):90-5. (PMID: 27193410)
Front Zool. 2021 Dec 4;18(1):60. (PMID: 34863212)
Nucleic Acids Res. 2012 Aug;40(15):e115. (PMID: 22730293)
Nat Methods. 2021 Apr;18(4):366-368. (PMID: 33828273)
Insects. 2022 Mar 31;13(4):. (PMID: 35447785)
Commun Biol. 2024 Dec 2;7(1):1604. (PMID: 39623057)
Insect Mol Biol. 2013 Dec;22(6):635-47. (PMID: 23937246)
Insect Sci. 2025 Jun;32(3):899-911. (PMID: 39185918)
PLoS One. 2016 Aug 25;11(8):e0161667. (PMID: 27560151)
BMC Mol Biol. 2019 Feb 18;20(1):6. (PMID: 30777032)
BMC Genomics. 2015 Aug 26;16:635. (PMID: 26306628)
PLoS One. 2011;6(7):e21800. (PMID: 21789182)
J Agric Food Chem. 2023 May 17;71(19):7201-7209. (PMID: 37146201)
Insects. 2023 Nov 18;14(11):. (PMID: 37999092)
F1000Res. 2015 Dec 30;4:1521. (PMID: 26925227)
Insect Sci. 2023 Oct;30(5):1309-1324. (PMID: 36763354)
Insect Mol Biol. 2016 Jun;25(3):315-23. (PMID: 26959524)
Nucleic Acids Res. 2011 Nov 1;39(20):8703-11. (PMID: 21764773)
Insects. 2020 Aug 20;11(9):. (PMID: 32825516)
Bioinformatics. 2016 Oct 1;32(19):3047-8. (PMID: 27312411)
G3 (Bethesda). 2021 Oct 19;11(11):. (PMID: 34557910)
Bioinformatics. 2010 Jan 1;26(1):139-40. (PMID: 19910308)
Curr Opin Insect Sci. 2015 Oct;11:39-46. (PMID: 28285758)
Front Genet. 2022 Jun 23;13:880000. (PMID: 35812742)
Nat Methods. 2012 Mar 04;9(4):357-9. (PMID: 22388286)
Sci Rep. 2018 Feb 1;8(1):2061. (PMID: 29391456)
Insect Sci. 2022 Apr;29(2):344-362. (PMID: 34388292)
J Econ Entomol. 2016 Aug;109(4):1914-21. (PMID: 27329631)
Arch Insect Biochem Physiol. 2025 Jun;119(2):e70069. (PMID: 40497425)
Front Plant Sci. 2021 Jan 28;11:526455. (PMID: 33584737)
Annu Rev Entomol. 2018 Jan 7;63:489-511. (PMID: 29058980)
Front Plant Sci. 2022 Apr 01;13:804839. (PMID: 35432425)
Mol Biol Rep. 2021 May;48(5):4383-4396. (PMID: 34091816)
Pest Manag Sci. 2021 May;77(5):2188-2196. (PMID: 33099867)
Nature. 2003 Sep 25;425(6956):415-9. (PMID: 14508493)
PLoS Negl Trop Dis. 2023 Jun 26;17(6):e0011411. (PMID: 37363930)
Front Cell Dev Biol. 2023 Jul 27;11:1190258. (PMID: 37576597)
Sci Rep. 2024 Oct 18;14(1):24451. (PMID: 39424855)
Mol Biol Evol. 2021 Jun 25;38(7):3022-3027. (PMID: 33892491)
Cell Rep. 2017 Jan 17;18(3):762-776. (PMID: 28099853)
Pest Manag Sci. 2019 Jan;75(1):18-28. (PMID: 29931761)
J Econ Entomol. 2020 Feb 8;113(1):115-125. (PMID: 31560771)
Methods. 2001 Dec;25(4):402-8. (PMID: 11846609)
BMC Bioinformatics. 2008 Dec 29;9:559. (PMID: 19114008)
PLoS One. 2023 Jan 3;18(1):e0279983. (PMID: 36595511)
Nat Rev Genet. 2011 Sep 07;12(10):671-82. (PMID: 21897427)
Bioinformatics. 2017 Sep 15;33(18):2938-2940. (PMID: 28645171)
BMC Genomics. 2019 Dec 9;20(1):954. (PMID: 31818245)
Arch Insect Biochem Physiol. 2019 Feb;100(2):e21522. (PMID: 30484903)
Nucleic Acids Res. 2000 Jan 1;28(1):27-30. (PMID: 10592173)
Bull Entomol Res. 2004 Jun;94(3):189-200. (PMID: 15191620)
Nat Methods. 2017 Apr;14(4):417-419. (PMID: 28263959)
Genomics. 2020 Mar;112(2):1651-1659. (PMID: 31626898)
Grant Information:
88887.924861/2023-00 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; 2023/14813-5 Fundação de Amparo à Pesquisa do Estado de São Paulo; 22/04006-2 Fundação de Amparo à Pesquisa do Estado de São Paulo; 170909/2023-9 Conselho Nacional de Desenvolvimento Científico e Tecnológico; 20.18.01.014.00.00 Empresa Brasileira de Pesquisa Agropecuária
Contributed Indexing:
Keywords: Coexpression network; Gene silencing; Hemiptera; Poaceae; RNA-seq
Entry Date(s):
Date Created: 20260106 Date Completed: 20260203 Latest Revision: 20260206
Update Code:
20260206
PubMed Central ID:
PMC12870070
DOI:
10.1186/s12864-025-12447-3
PMID:
41491413
Database:
MEDLINE
*Further Information*
*Mahanarva fimbriolata (Distant 1909) (Hemiptera: Cercopidae) is a major pest that reduces forage production in Brazil, yet few viable control alternatives exist. The RNA interference (RNAi) gene silencing method is a specific and efficient approach that may aid the development of a sustainable management strategy for this pest. It is known that some genes involved in the RNAi machinery are lacking in certain Hemiptera species; therefore, verifying their presence in each target species is necessary. Here, transcriptome assembly of Mahanarva fimbriolata at different developmental stages (egg, nymph and adult stages) was performed, differentially expressed genes were identified, RNAi-related genes described in the literature were annotated in the transcriptome, and coexpression network modeling for the identification of potential RNAi targets was performed. The analysis revealed that the most significant differences in gene expression were between samples in the egg stage and samples in the other development stages. Enriched Gene Ontology terms related to insect growth (e.g., cell division, metamorphosis and flight) and corresponding pathways (e.g., DNA replication and glycolysis/gluconeogenesis) were identified. Coexpression networks demonstrated the importance of biosynthetic hormone processes within specific modules and revealed potential silencing targets, including hub genes such as RPB7 and Talin-2. Transcript annotation and analysis revealed more than 20 genes related to five major RNAi-related processes and factors (dsRNA cleavage, endonucleases, dsRNA binding, dsRNA transport and uptake, and auxiliary RISC-associated or regulatory factors). This work provides a comprehensive molecular overview of metamorphosis in M. fimbriolata, confirms the presence of active RNAi machinery, and reveals potential targets for future gene silencing approaches.
(© 2026. The Author(s).)*
*Declarations. Ethical approval: Not applicable. Competing interests: The authors declare no competing interests.*