• Whole-transcriptome sequencing...

*Result*: Whole-transcriptome sequencing revealed the ceRNA regulatory network of stable knockdown of the CD8A gene in chicken T lymphocytes.

Title:
Whole-transcriptome sequencing revealed the ceRNA regulatory network of stable knockdown of the CD8A gene in chicken T lymphocytes.
Authors:
Du Y; College of Agronomy and Life Sciences, Kunming University, Kunming, Yunnan 650200, China., Zhang R; Yunnan Academy of Animal Husbandry and Veterinary Sciences, Institute of Poultry Science, Kunming, Yunnan 650000, China., Li M; College of Agronomy and Life Sciences, Kunming University, Kunming, Yunnan 650200, China., Wang X; College of Agronomy and Life Sciences, Kunming University, Kunming, Yunnan 650200, China., Zhang H; College of Agronomy and Life Sciences, Kunming University, Kunming, Yunnan 650200, China., Zhang B; College of Agronomy and Life Sciences, Kunming University, Kunming, Yunnan 650200, China., Liao B; College of Agronomy and Life Sciences, Kunming University, Kunming, Yunnan 650200, China., Wang K; Yunnan Provincial Key Laboratory of Animal Nutrition and Feed, Yunnan Agricultural University, Kunming, Yunnan 650201, China., Zi X; Yunnan Provincial Key Laboratory of Animal Nutrition and Feed, Yunnan Agricultural University, Kunming, Yunnan 650201, China., Huang T; College of Agronomy and Life Sciences, Kunming University, Kunming, Yunnan 650200, China., Ge C; Yunnan Provincial Key Laboratory of Animal Nutrition and Feed, Yunnan Agricultural University, Kunming, Yunnan 650201, China., Ma J; Yunnan Academy of Animal Husbandry and Veterinary Sciences, Institute of Poultry Science, Kunming, Yunnan 650000, China., Li K; Yunnan Academy of Animal Husbandry and Veterinary Sciences, Institute of Poultry Science, Kunming, Yunnan 650000, China., Xin A; Yunnan Academy of Animal Husbandry and Veterinary Sciences, Institute of Poultry Science, Kunming, Yunnan 650000, China. Electronic address: aiguo_xin@hotmail.com.
Source:
Poultry science [Poult Sci] 2026 Feb; Vol. 105 (2), pp. 106318. Date of Electronic Publication: 2025 Dec 19.
Publication Type:
Journal Article
Language:
English
Journal Info:
Publisher: Elsevier Country of Publication: England NLM ID: 0401150 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1525-3171 (Electronic) Linking ISSN: 00325791 NLM ISO Abbreviation: Poult Sci Subsets: MEDLINE
Imprint Name(s):
Publication: 2020- : [Cambridge, UK] : Elsevier
Original Publication: Champaign Il : Poultry Science Association
MeSH Terms:
Chickens*/genetics , Chickens*/immunology , T-Lymphocytes*/metabolism , CD8 Antigens*/genetics , CD8 Antigens*/metabolism , Avian Proteins*/genetics , Avian Proteins*/metabolism , Transcriptome* , Gene Regulatory Networks*, Gene Expression Profiling/veterinary ; Gene Knockdown Techniques/veterinary ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Sequence Analysis, RNA/veterinary ; Animals ; RNA, Competitive Endogenous
Contributed Indexing:
Keywords: CD8A; Interference; T lymphocytes; Whole-transcriptome analysis; ceRNA network
Substance Nomenclature:
0 (CD8 Antigens)
0 (Avian Proteins)
0 (CD8 antigen, alpha chain)
0 (MicroRNAs)
0 (RNA, Competitive Endogenous)
Entry Date(s):
Date Created: 20251226 Date Completed: 20260305 Latest Revision: 20260305
Update Code:
20260306
PubMed Central ID:
PMC12800364
DOI:
10.1016/j.psj.2025.106318
PMID:
41453319
Database:
MEDLINE

*Further Information*

*CD8 subunit alpha (CD8A) is an important gene in immunity and is involved in the functional regulation of T lymphocytes. Previous studies have confirmed that interfering with CD8A expression inhibits the proliferation and induces the apoptosis of T lymphocytes. However, the specific regulatory mechanism of CD8A in chicken T lymphocytes remains unreported. In this study, whole-transcriptome RNA sequencing analyses of chicken T lymphocytes with interference of CD8A were performed. The whole-transcriptome RNA sequencing results revealed 465 differentially expressed genes (DEGs), 108 differentially expressed miRNAs (DEMs), 679 differentially expressed lncRNAs (DELs), and 18 differentially expressed circRNAs (DECs). The competitive endogenous RNA (ceRNA) regulatory network analysis indicated that interference with CD8A may regulate "interleukin-8 receptor binding", "positive regulation of the cellular defense response", "glycosaminoglycan biosynthesis-keratan sulfate", "glycosphingolipid biosynthesis-ganglio series", etc., thereby regulating the development of chicken T lymphocytes and immunity. Moreover, we extracted eight genes that were significantly enriched in the GO and KEGG analyses, including MITF, ARID5B, IL8L2, RUNX1, JAK1, MKNK2, TIPARP, and ST3GAL1, which are considered to be immune genes that are regulated by miR-1306-5p, miR-24-3p_R+1, miR-2478_L-1_1ss2TG, miR-106a-5p, and miR-17-5p. These miRNAs are competitively combined with other lncRNAs, including MSTRG.3287.1, MSTRG.851.3, MSTRG.5709.2, MSTRG.8606.10, MSTRG.8606.9, MSTRG.6504.2, and MSTRG.2145.4, etc., via circRNAs, including cirRNA145 and cirRNA147. Therefore, CD8A may affect T-cell development and thereby influence the immunity of chickens through these ceRNA regulatory networks and signaling pathways.
(Copyright © 2025. Published by Elsevier Inc.)*

*Disclosures The authors declare that they have no conflicts of interest.*