• Transcriptional Regulation of...

*Result*: Transcriptional Regulation of Nuclear Receptor NlNR2E in Response to Multiple Insecticides in Nilaparvata lugens.

Title:
Transcriptional Regulation of Nuclear Receptor NlNR2E in Response to Multiple Insecticides in Nilaparvata lugens.
Authors:
Lin K; State Key Laboratory of Green Pesticide, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China.; Guangxi Key Laboratory of Agric-Environment and Agric-Products Safety, College of Agriculture, Guangxi University, Nanning 530004, China., Zhong Z; State Key Laboratory of Green Pesticide, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China., Hu Y; State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China., Wu H; State Key Laboratory of Green Pesticide, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China., Guo Y; State Key Laboratory of Green Pesticide, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China., Huang S; State Key Laboratory of Green Pesticide, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China., Wu H; School of Life Science and Technology, Wuhan Polytechnic University, Wuhan 430023, China., Xu X; State Key Laboratory of Green Pesticide, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China., Jin F; State Key Laboratory of Green Pesticide, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China., Pang R; State Key Laboratory of Green Pesticide, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China.
Source:
Journal of agricultural and food chemistry [J Agric Food Chem] 2026 Jan 14; Vol. 74 (1), pp. 1515-1524. Date of Electronic Publication: 2025 Dec 26.
Publication Type:
Journal Article
Language:
English
Journal Info:
Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5118 (Electronic) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE
Imprint Name(s):
Original Publication: Washington, American Chemical Society.
MeSH Terms:
Hemiptera*/genetics , Hemiptera*/drug effects , Hemiptera*/metabolism , Insecticides*/pharmacology , Insect Proteins*/genetics , Insect Proteins*/metabolism , Receptors, Cytoplasmic and Nuclear*/genetics , Receptors, Cytoplasmic and Nuclear*/metabolism, Gene Expression Regulation/drug effects ; ATP-Binding Cassette Transporters/genetics ; ATP-Binding Cassette Transporters/metabolism ; Animals ; Insecticide Resistance ; Promoter Regions, Genetic ; RNA Interference
Contributed Indexing:
Keywords: ATP-binding cassette transporter; Nilaparvata lugens; multiple insecticide resistance; nuclear receptor; transcription regulation
Substance Nomenclature:
0 (Insecticides)
0 (Insect Proteins)
0 (Receptors, Cytoplasmic and Nuclear)
0 (ATP-Binding Cassette Transporters)
Entry Date(s):
Date Created: 20251226 Date Completed: 20260115 Latest Revision: 20260115
Update Code:
20260130
DOI:
10.1021/acs.jafc.5c08828
PMID:
41450265
Database:
MEDLINE

*Further Information*

*Insecticide resistance has led to the failure of chemical pest control and threatens global food security, with transcriptional regulation of detoxification genes by xenobiotic-response transcription factors (TFs) playing a key role. However, the regulation mechanism remains unclear in most crop insect pests. This study characterized the role of the nuclear receptor NlNR2E in response to insecticides in the brown planthopper Nilaparvata lugens, a destructive rice pest. Using DAP-seq, we identified detoxification gene promoters targeted by NlNR2E. NlABCG23, encoding an ATP-binding cassette (ABC) transporter, showed significantly reduced expression after the RNA interference (RNAi) knockdown of NlNR2E. Dual-luciferase reporter, yeast one-hybrid, and electrophoretic mobility shift assays confirmed that NlNR2E directly binds to NlABCG23 promoter, thus activating NlABCG23 expression. RNAi knockdown of NlABCG23 significantly reduced resistance to imidacloprid, dinotefuran, pymetrozine, and chlorpyrifos in N. lugens. These findings illustrated how xenobiotic-induced TF regulates the ABC transporter, conferring resistance to multiple insecticides in planthoppers.*