• CYP321A9 mediates the metaboli...

*Result*: CYP321A9 mediates the metabolism of two diamide insecticides, chlorantraniliprole and tetrachlorantraniliprole in Spodoptera frugiperda.

Title:
CYP321A9 mediates the metabolism of two diamide insecticides, chlorantraniliprole and tetrachlorantraniliprole in Spodoptera frugiperda.
Authors:
Cao W; Guangxi Key Laboratory of Agric-Environment and Agric-products Safety, Guangxi University, Nanning, Guangxi 530004, China., Huang M; Guangxi Key Laboratory of Agric-Environment and Agric-products Safety, Guangxi University, Nanning, Guangxi 530004, China., Zhang Y; Guangxi Key Laboratory of Agric-Environment and Agric-products Safety, Guangxi University, Nanning, Guangxi 530004, China., Long M; Guangxi Key Laboratory of Agric-Environment and Agric-products Safety, Guangxi University, Nanning, Guangxi 530004, China., Li X; Guangxi Key Laboratory of Agric-Environment and Agric-products Safety, Guangxi University, Nanning, Guangxi 530004, China. Electronic address: lxsnngx@gxu.edu.cn.
Source:
Pesticide biochemistry and physiology [Pestic Biochem Physiol] 2026 Jan; Vol. 216 (Pt 2), pp. 106819. Date of Electronic Publication: 2025 Nov 07.
Publication Type:
Journal Article
Language:
English
Journal Info:
Publisher: Academic Press Country of Publication: United States NLM ID: 1301573 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9939 (Electronic) Linking ISSN: 00483575 NLM ISO Abbreviation: Pestic Biochem Physiol Subsets: MEDLINE
Imprint Name(s):
Original Publication: New York Ny : Academic Press
MeSH Terms:
Spodoptera*/metabolism , Spodoptera*/drug effects , Spodoptera*/genetics , Spodoptera*/enzymology , ortho-Aminobenzoates*/metabolism , ortho-Aminobenzoates*/pharmacology , Insecticides*/metabolism , Insecticides*/pharmacology , Cytochrome P-450 Enzyme System*/metabolism , Cytochrome P-450 Enzyme System*/genetics , Insect Proteins*/metabolism , Insect Proteins*/genetics, Insecticide Resistance/genetics ; Animals ; Molecular Docking Simulation
Contributed Indexing:
Keywords: CYP321A9; Chlorantraniliprole; Metabolic resistance; Spodoptera frugiperda; Tetrachlorantraniliprole
Substance Nomenclature:
0 (ortho-Aminobenzoates)
622AK9DH9G (chlorantranilipole)
0 (Insecticides)
9035-51-2 (Cytochrome P-450 Enzyme System)
0 (Insect Proteins)
Entry Date(s):
Date Created: 20251205 Date Completed: 20251205 Latest Revision: 20251205
Update Code:
20260130
DOI:
10.1016/j.pestbp.2025.106819
PMID:
41350073
Database:
MEDLINE

*Further Information*

*The resistance of Spodoptera frugiperda to diamide insecticides poses significant challenges to agricultural production. This study investigated the effects of chlorantraniliprole (CHL) and tetrachlorantraniliprole (TET) on the expression of the cytochrome P450 genes SfCYP321A9 and SfCYP6AE44 in S. frugiperda and elucidated their roles in insecticide metabolism. Both insecticides significantly upregulated SfCYP321A9 and SfCYP6AE44 expression, with peak levels observed in the midgut. RNA interference-mediated downregulation of these genes markedly decreased the metabolic rates of CHL and TET, underscoring their key roles in insecticide metabolism. In vitro assays confirmed that the CYP321A9 protein directly metabolizes CHL and TET, and metabolite profiling clarified the associated metabolic pathways and product profiles. Molecular docking further revealed the specific binding patterns between CYP321A9 and the two insecticides. Collectively, this study provides direct evidence of the involvement of SfCYP321A9 in bisamide insecticide metabolism, emphasizing its role in S. frugiperda resistance. These findings offer novel insights into insecticide resistance and cross-resistance mechanisms and underpin the development of enhanced pest control strategies.
(Copyright © 2025. Published by Elsevier Inc.)*

*Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.*