*Result*: Visual detection of seizures in mice using supervised machine learning.
Title:
Visual detection of seizures in mice using supervised machine learning.
Authors:
Sabnis GS; The Jackson Laboratory, Bar Harbor, ME 04609, USA., Hession L; The Jackson Laboratory, Bar Harbor, ME 04609, USA., Mahoney JM; The Jackson Laboratory, Bar Harbor, ME 04609, USA., Mobley A; The Jackson Laboratory, Bar Harbor, ME 04609, USA., Santos M; The Jackson Laboratory, Bar Harbor, ME 04609, USA., Geuther B; The Jackson Laboratory, Bar Harbor, ME 04609, USA., Kumar V; The Jackson Laboratory, Bar Harbor, ME 04609, USA; School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, MA 02111, USA; Graduate School of Biomedical Sciences and Engineering, University of Maine, Orono, ME 04469, USA. Electronic address: vivek.kumar@jax.org.
Source:
Cell reports methods [Cell Rep Methods] 2025 Dec 15; Vol. 5 (12), pp. 101242. Date of Electronic Publication: 2025 Nov 26.
Publication Type:
Journal Article
Language:
English
Journal Info:
Publisher: Elsevier Inc Country of Publication: United States NLM ID: 9918227360606676 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2667-2375 (Electronic) Linking ISSN: 26672375 NLM ISO Abbreviation: Cell Rep Methods Subsets: MEDLINE
Imprint Name(s):
Original Publication: [New York] : Elsevier Inc., [2021]-
MeSH Terms:
Comments:
Update of: bioRxiv. 2024 May 30:2024.05.29.596520. doi: 10.1101/2024.05.29.596520.. (PMID: 38868170)
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J Clin Neurophysiol. 2006 Dec;23(6):521-31. (PMID: 17143140)
Nat Rev Dis Primers. 2018 May 03;4:18024. (PMID: 29722352)
Cell Rep. 2022 Jan 11;38(2):110231. (PMID: 35021077)
Mol Pain. 2020 Jan-Dec;16:1744806920958596. (PMID: 32955381)
Epilepsia. 2023 Jun;64(6):1653-1662. (PMID: 37013671)
Neuropsychiatr Dis Treat. 2014 Sep 09;10:1693-705. (PMID: 25228809)
Elife. 2021 Mar 17;10:. (PMID: 33729153)
Toxicol Sci. 2021 Jan 6;179(1):3-13. (PMID: 33165543)
Commun Biol. 2019 Mar 29;2:124. (PMID: 30937403)
IEEE Trans Biomed Eng. 2012 Dec;59(12):3379-85. (PMID: 22949042)
J Am Assoc Lab Anim Sci. 2012;51(5):600-8. (PMID: 23312089)
Exp Biol Med (Maywood). 2018 Sep;243(13):1037-1045. (PMID: 30253665)
Nat Methods. 2013 Jan;10(1):64-7. (PMID: 23202433)
Epilepsia. 2024 May;65(5):1176-1202. (PMID: 38426252)
Physiol Behav. 2009 Dec 7;98(5):579-86. (PMID: 19772866)
Genomics. 2000 Nov 15;70(1):62-5. (PMID: 11087662)
Nat Rev Genet. 2022 Sep;23(9):524-532. (PMID: 35354965)
PLoS Biol. 2019 Nov 21;17(11):e3000516. (PMID: 31751328)
Epilepsia Open. 2018 Oct 24;3(Suppl Suppl 2):114-119. (PMID: 30564769)
Epilepsy Behav. 2016 Jul;60:197-201. (PMID: 27208925)
Nature. 2013 Sep 12;501(7466):217-21. (PMID: 23934111)
Am J Hum Genet. 2019 Aug 1;105(2):267-282. (PMID: 31327507)
Aging Dis. 2022 Feb 1;13(1):215-231. (PMID: 35111370)
Epilepsy Behav. 2018 May;82:17-24. (PMID: 29574299)
Geroscience. 2025 Aug;47(4):5435-5448. (PMID: 40095188)
J Stat Softw. 2021 Sep;99(6):. (PMID: 34512213)
Epilepsia. 2025 Sep;66 Suppl 3:39-52. (PMID: 37983589)
Neuron. 2015 Dec 16;88(6):1121-1135. (PMID: 26687221)
Am J Physiol Regul Integr Comp Physiol. 2004 May;286(5):R967-74. (PMID: 14726427)
Sleep. 2022 Feb 14;45(2):. (PMID: 34718812)
Neurosci Biobehav Rev. 2024 Feb;157:105528. (PMID: 38160721)
Bio Protoc. 2020 Feb 20;10(4):e3533. (PMID: 33654757)
Commun Biol. 2022 Nov 18;5(1):1267. (PMID: 36400882)
Neural Plast. 2016;2016:8213878. (PMID: 26819775)
Nat Methods. 2024 Jul;21(7):1329-1339. (PMID: 38997595)
Annu Rev Genomics Hum Genet. 2020 Aug 31;21:205-230. (PMID: 32339036)
Seizure. 2020 Jan 24;76:72-78. (PMID: 32035366)
Clin Neurophysiol. 2006 Jul;117(7):1585-94. (PMID: 16684619)
J R Soc Interface. 2014 Oct 6;11(99):. (PMID: 25142523)
Nat Commun. 2018 Sep 3;9(1):3561. (PMID: 30177815)
Epilepsy Behav. 2024 May;154:109735. (PMID: 38522192)
Sci Rep. 2021 Apr 5;11(1):7482. (PMID: 33820942)
Neuron. 2020 Oct 14;108(1):193-208.e9. (PMID: 32853550)
IEEE Trans Pattern Anal Mach Intell. 2021 Oct;43(10):3349-3364. (PMID: 32248092)
Nat Commun. 2021 Aug 31;12(1):5188. (PMID: 34465784)
Nat Rev Neurol. 2014 May;10(5):283-92. (PMID: 24733163)
Epilepsy Behav. 2019 Jun;95:51-55. (PMID: 31026782)
Br J Pharmacol Chemother. 1960 Dec;15(4):540-3. (PMID: 19108140)
Epilepsia. 2006 Jun;47(6):966-80. (PMID: 16822243)
Neuron. 2014 Oct 1;84(1):18-31. (PMID: 25277452)
Nat Aging. 2022 Aug;2(8):756-766. (PMID: 37091193)
JAMA Neurol. 2022 Dec 1;79(12):1267-1276. (PMID: 36315135)
Neuron. 2023 May 3;111(9):1440-1452.e5. (PMID: 36841241)
Proc Natl Acad Sci U S A. 2011 Sep 13;108 Suppl 3:15557-64. (PMID: 21896739)
Clin Neurophysiol. 2021 May;132(5):1173-1184. (PMID: 33678577)
Epilepsia. 2020 Nov;61 Suppl 1:S36-S40. (PMID: 32378204)
Nat Rev Neurol. 2023 Feb;19(2):69-70. (PMID: 36522546)
Nat Neurosci. 2020 Dec;23(12):1537-1549. (PMID: 33169033)
Lancet Neurol. 2017 Feb;16(2):135-143. (PMID: 28102150)
Epilepsia. 2023 Dec;64 Suppl 4:S65-S71. (PMID: 35194778)
Sci Rep. 2022 Nov 15;12(1):19571. (PMID: 36379994)
Electroencephalogr Clin Neurophysiol. 1972 Mar;32(3):281-94. (PMID: 4110397)
Nat Genet. 2023 Sep;55(9):1471-1482. (PMID: 37653029)
J Clin Neurophysiol. 2006 Dec;23(6):521-31. (PMID: 17143140)
Nat Rev Dis Primers. 2018 May 03;4:18024. (PMID: 29722352)
Cell Rep. 2022 Jan 11;38(2):110231. (PMID: 35021077)
Mol Pain. 2020 Jan-Dec;16:1744806920958596. (PMID: 32955381)
Epilepsia. 2023 Jun;64(6):1653-1662. (PMID: 37013671)
Neuropsychiatr Dis Treat. 2014 Sep 09;10:1693-705. (PMID: 25228809)
Elife. 2021 Mar 17;10:. (PMID: 33729153)
Toxicol Sci. 2021 Jan 6;179(1):3-13. (PMID: 33165543)
Commun Biol. 2019 Mar 29;2:124. (PMID: 30937403)
IEEE Trans Biomed Eng. 2012 Dec;59(12):3379-85. (PMID: 22949042)
J Am Assoc Lab Anim Sci. 2012;51(5):600-8. (PMID: 23312089)
Exp Biol Med (Maywood). 2018 Sep;243(13):1037-1045. (PMID: 30253665)
Nat Methods. 2013 Jan;10(1):64-7. (PMID: 23202433)
Contributed Indexing:
Keywords: CP: computational biology; CP: neuroscience; computer vision; epilepsy; high throughput; machine learning; mouse models; open field; seizure; supervised learning
Substance Nomenclature:
WM5Z385K7T (Pentylenetetrazole)
Entry Date(s):
Date Created: 20251127 Date Completed: 20251216 Latest Revision: 20260203
Update Code:
20260203
PubMed Central ID:
PMC12859513
DOI:
10.1016/j.crmeth.2025.101242
PMID:
41308647
Database:
MEDLINE
*Further Information*
*Seizures are caused by abnormal synchronous brain activity. The resulting changes in muscle tone, such as twitching, stiffness, or jerking, are used in visual scoring systems such as the Racine scale to quantify seizure intensity. However, visual inspection is time consuming, low throughput, and partially subjective, and there is a need for scalable and rigorous quantitative approaches. We used supervised machine learning approaches to develop automated classifiers to predict seizure severity directly from non-invasive video data. Using the pentylenetetrazole (PTZ)-induced seizure model in mice, we trained video-only classifiers to predict ictal events and combined these events to predict composite seizure intensity for a recording session, as well as time-localized seizure intensity scores. Our results show that seizure events and overall intensity can be rigorously quantified directly from overhead video of mice in a standard open field using supervised approaches. These results enable high-throughput, non-invasive, and standardized seizure scoring for neurogenetics and therapeutic discovery.
(Copyright © 2025 The Authors. Published by Elsevier Inc. All rights reserved.)*
*Declaration of interests The authors declare no competing interests.*