• Transcriptomic insights into T...

*Result*: Transcriptomic insights into TiO 2 nanoparticle-assisted enhancement of growth, lipid production, and dark fermentation wastewater treatment in Chlorella sp. DT025.

Title:
Transcriptomic insights into TiO 2 nanoparticle-assisted enhancement of growth, lipid production, and dark fermentation wastewater treatment in Chlorella sp. DT025.
Authors:
Wu X; College of Biological and Food Engineering, Anhui Polytechnic University, Wuhu, Anhui 241000, China., Xu J; College of Biological and Food Engineering, Anhui Polytechnic University, Wuhu, Anhui 241000, China., He B; College of Biological and Food Engineering, Anhui Polytechnic University, Wuhu, Anhui 241000, China; Anhui Engineering Laboratory for Industrial Microbiology Molecular Breeding, Anhui Polytechnic University, Wuhu, Anhui 241000, China., Zhang Q; College of Biological and Food Engineering, Anhui Polytechnic University, Wuhu, Anhui 241000, China; Anhui Engineering Laboratory for Industrial Microbiology Molecular Breeding, Anhui Polytechnic University, Wuhu, Anhui 241000, China., Sheng Y; College of Biological and Food Engineering, Anhui Polytechnic University, Wuhu, Anhui 241000, China; Anhui Engineering Laboratory for Industrial Microbiology Molecular Breeding, Anhui Polytechnic University, Wuhu, Anhui 241000, China. Electronic address: shengyequan@mail.ahpu.edu.cn., Tan X; College of Biological and Food Engineering, Anhui Polytechnic University, Wuhu, Anhui 241000, China; Anhui Engineering Laboratory for Industrial Microbiology Molecular Breeding, Anhui Polytechnic University, Wuhu, Anhui 241000, China., Li Y; College of Biological and Food Engineering, Anhui Polytechnic University, Wuhu, Anhui 241000, China; Anhui Engineering Laboratory for Industrial Microbiology Molecular Breeding, Anhui Polytechnic University, Wuhu, Anhui 241000, China. Electronic address: lyb@ahpu.edu.cn.
Source:
Bioresource technology [Bioresour Technol] 2026 Feb; Vol. 441, pp. 133625. Date of Electronic Publication: 2025 Nov 07.
Publication Type:
Journal Article
Language:
English
Journal Info:
Publisher: Elsevier Applied Science Country of Publication: England NLM ID: 9889523 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2976 (Electronic) Linking ISSN: 09608524 NLM ISO Abbreviation: Bioresour Technol Subsets: MEDLINE
Imprint Name(s):
Original Publication: Barking, Essex, England : New York, N.Y. : Elsevier Applied Science ; Elsevier Science Pub. Co., 1991-
MeSH Terms:
Titanium*/pharmacology , Titanium*/chemistry , Chlorella*/growth & development , Chlorella*/metabolism , Chlorella*/genetics , Chlorella*/drug effects , Wastewater*/microbiology , Wastewater*/chemistry , Fermentation*/drug effects , Nanoparticles*/chemistry , Lipids*/biosynthesis , Transcriptome*/drug effects , Water Purification*/methods, Biological Oxygen Demand Analysis ; Nitrogen ; Biomass ; Darkness ; Microalgae ; Lipid Metabolism/drug effects
Contributed Indexing:
Keywords: Biomass; Enzyme activity; Lipid production; Transcriptome analysis
Substance Nomenclature:
D1JT611TNE (Titanium)
15FIX9V2JP (titanium dioxide)
0 (Wastewater)
0 (Lipids)
N762921K75 (Nitrogen)
Entry Date(s):
Date Created: 20251108 Date Completed: 20251211 Latest Revision: 20251216
Update Code:
20260130
DOI:
10.1016/j.biortech.2025.133625
PMID:
41205631
Database:
MEDLINE

*Further Information*

*Straw biomass anaerobic hydrogen production generates dark fermentation hydrogen-producing wastewater (DFHW), whose high chemical oxygen demand (COD) and total nitrogen (TN) inhibit microalgal activity. This study added 50 mg·L <sup>-1</sup> 20 nm TiO <subscript>2</subscript> nanoparticles (NPs) to DFHW to enhance microalgal stress tolerance. With optimized with the addition of TiO <subscript>2</subscript> nanoparticles (TiO <subscript>2</subscript> NPs), Chlorella sp. DT025 reached 90.46 × 10 <sup>7</sup> cells·mL <sup>-1</sup> cell density and 2.91 g·L <sup>-1</sup> biomass. COD and TN removal rates were 68 % and 35 %, lipid production 0.62 g·L <sup>-1</sup> . The addition of TiO <subscript>2</subscript> NPs increased chlorophyll a and b by 19 % and 41 %. Transcriptome analysis showed upregulated genes for chlorophyll synthesis (chlH, chlL, chlN), photosynthesis (psaA, psbA, petA) and lipid accumulation (ACS, ACACA, ppc, rbcL). Overall, this study demonstrated that TiO <subscript>2</subscript> NPs synergistically improved microalgal growth, DFHW treatment efficiency and lipid synthesis, providing a feasible strategy for integrated wastewater valorization and bioresource production.
(Copyright © 2025 Elsevier Ltd. All rights reserved.)*

*Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.*