• Enhancement mechanism of coppe...

*Result*: Enhancement mechanism of copper on heterotrophic nitrification-aerobic denitrification by cold-adapted Pseudomonas sp. NY1: Insights from genome and transcriptome.

Title:
Enhancement mechanism of copper on heterotrophic nitrification-aerobic denitrification by cold-adapted Pseudomonas sp. NY1: Insights from genome and transcriptome.
Authors:
Liao Y; Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou Key Laboratory of Agricultural Microbiology, College of Life Sciences, Guizhou University, Guiyang, Guizhou Province 550025, China., Yang L; Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou Key Laboratory of Agricultural Microbiology, College of Life Sciences, Guizhou University, Guiyang, Guizhou Province 550025, China., He T; Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou Key Laboratory of Agricultural Microbiology, College of Life Sciences, Guizhou University, Guiyang, Guizhou Province 550025, China; Key Laboratory of Karst Georesources and Environment (Guizhou University), Ministry of Education, Guizhou University, Guiyang, Guizhou Province 550025, China. Electronic address: txhe@gzu.edu.cn., Zheng C; Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou Key Laboratory of Agricultural Microbiology, College of Life Sciences, Guizhou University, Guiyang, Guizhou Province 550025, China., Zhang M; Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou Key Laboratory of Agricultural Microbiology, College of Life Sciences, Guizhou University, Guiyang, Guizhou Province 550025, China., Lu L; Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou Key Laboratory of Agricultural Microbiology, College of Life Sciences, Guizhou University, Guiyang, Guizhou Province 550025, China.
Source:
Bioresource technology [Bioresour Technol] 2026 Feb; Vol. 441, pp. 133565. Date of Electronic Publication: 2025 Oct 28.
Publication Type:
Journal Article
Language:
English
Journal Info:
Publisher: Elsevier Applied Science Country of Publication: England NLM ID: 9889523 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2976 (Electronic) Linking ISSN: 09608524 NLM ISO Abbreviation: Bioresour Technol Subsets: MEDLINE
Imprint Name(s):
Original Publication: Barking, Essex, England : New York, N.Y. : Elsevier Applied Science ; Elsevier Science Pub. Co., 1991-
MeSH Terms:
Copper*/pharmacology , Denitrification*/drug effects , Pseudomonas*/genetics , Pseudomonas*/drug effects , Pseudomonas*/metabolism , Cold Temperature* , Nitrification*/drug effects , Transcriptome*/genetics , Transcriptome*/drug effects , Heterotrophic Processes*/drug effects , Genome, Bacterial*/genetics , Adaptation, Physiological*/drug effects, Aerobiosis/drug effects ; Nitrites/metabolism ; Nitrogen/metabolism ; Ammonium Compounds ; Wastewater
Contributed Indexing:
Keywords: Copper stress; Low-temperature; Nitrogen metabolism genes; Nitrogen removal
Substance Nomenclature:
789U1901C5 (Copper)
0 (Nitrites)
N762921K75 (Nitrogen)
0 (Ammonium Compounds)
0 (Wastewater)
Entry Date(s):
Date Created: 20251030 Date Completed: 20251211 Latest Revision: 20251216
Update Code:
20260130
DOI:
10.1016/j.biortech.2025.133565
PMID:
41167485
Database:
MEDLINE

*Further Information*

*Low concentrations of copper ions (Cu <sup>2+</sup> ) can promote the heterotrophic nitrification-aerobic denitrification (HN-AD) ability of cold-adapted Pseudomonas sp. NY1, but the exact promotion mechanisms remain unclear. In this study, nitrogen balance data revealed that the removal rates of NO <subscript>2</subscript><sup>-</sup> -N and total nitrogen increased by 0.37 mg/L/h and 0.28 mg/L/h after addition of 0.05 mg/L Cu <sup>2+</sup> . The genetic bases for cold-adapted, copper-resistant, and multiple-nitrogen metabolism pathways of strain NY1 were determined by whole genome sequencing. Transcriptome and reverse transcription-quantitative polymerase chain reaction analysis revealed that strain NY1 promoted amino acid and energy production by the upregulation of assimilatory nitrite reduction to ammonium in response to copper stress. These results provided insights into the positive effects of Cu <sup>2+</sup> on HN-AD bacteria by accelerating the removal of ammonium and nitrite, and advancing the application and development of HN-AD bacteria in copper-containing and low-temperature wastewater.
(Copyright © 2025 Elsevier Ltd. All rights reserved.)*

*Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.*