• Transcriptome analysis and exp...

*Result*: Transcriptome analysis and exploration of immune key genes of hepatopancreas after Poly(I:C) stimulation in Amphioctopus fangsiao.

Title:
Transcriptome analysis and exploration of immune key genes of hepatopancreas after Poly(I:C) stimulation in Amphioctopus fangsiao.
Authors:
Huang X; School of Agriculture, Ludong University, Yantai, 264025, China., Gu J; Binzhou Testing Center, Binzhou, 256600, China., Huang Z; School of Agriculture, Ludong University, Yantai, 264025, China., Cui X; School of Agriculture, Ludong University, Yantai, 264025, China., Qiu D; School of Agriculture, Ludong University, Yantai, 264025, China., Li Z; School of Agriculture, Ludong University, Yantai, 264025, China., Feng Y; School of Agriculture, Ludong University, Yantai, 264025, China., Xu X; School of Agriculture, Ludong University, Yantai, 264025, China. Electronic address: xxh83121@163.com., Yang J; School of Agriculture, Ludong University, Yantai, 264025, China. Electronic address: ladderup@126.com.
Source:
Comparative biochemistry and physiology. Part D, Genomics & proteomics [Comp Biochem Physiol Part D Genomics Proteomics] 2026 Mar; Vol. 57, pp. 101665. Date of Electronic Publication: 2025 Oct 23.
Publication Type:
Journal Article
Language:
English
Journal Info:
Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101270611 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-0407 (Electronic) Linking ISSN: 1744117X NLM ISO Abbreviation: Comp Biochem Physiol Part D Genomics Proteomics Subsets: MEDLINE
Imprint Name(s):
Original Publication: Amsterdam : Elsevier, 2006-
MeSH Terms:
Poly I-C*/pharmacology , Hepatopancreas*/immunology , Hepatopancreas*/drug effects , Hepatopancreas*/metabolism , Transcriptome*, Animals ; Gene Expression Profiling ; Immunity, Innate
Contributed Indexing:
Keywords: Amphioctopus fangsiao; Hepatopancreas; Immune response; Poly(I:C); Protein-protein interaction network
Substance Nomenclature:
O84C90HH2L (Poly I-C)
Entry Date(s):
Date Created: 20251030 Date Completed: 20251210 Latest Revision: 20251210
Update Code:
20260130
DOI:
10.1016/j.cbd.2025.101665
PMID:
41166849
Database:
MEDLINE

*Further Information*

*Amphioctopus fangsiao is a commercially valuable cephalopod species prized for its distinctive flavor and nutritional profile. However, its large-scale aquaculture is hampered by significant challenges, particularly outbreaks of infectious diseases. Viral pathogens, which can be co-transmitted among marine species, pose a severe threat to sustainable population development. To elucidate the antiviral defense mechanisms in A. fangsiao, we conducted transcriptomic profiling of hepatopancreatic tissue following stimulation with polyinosinic-polycytidylic acid (Poly(I:C)), a well-established viral mimic known to activate innate immune pathways. Comparative transcriptomic profiling of the hepatopancreas-a key immune organ-at 6 h and 24 h post-stimulation identified 1406 and 904 differentially expressed genes (DEGs), respectively. Functional enrichment analysis revealed that these DEGs were significantly involved in critical immune-related pathways, including apoptosis, ECM-receptor interaction, and the MAPK and Hippo signaling pathways. Furthermore, by integrating protein-protein interaction (PPI) network analysis with KEGG pathway data, we identified 18 hub genes-such as TNFSF10, JUN, EGFR, and CTSB-that were potential key regulators of the antiviral response. The expression patterns of the aforementioned hub genes were successfully validated by means of quantitative reverse transcription polymerase chain reaction (qRT-PCR). This study comprehensively revealed the transcriptome characteristics of antiviral immune response of A. fangsiao for the first time. Our findings not only advance the understanding of innate immunity in cephalopods but also offer valuable genetic resources and potential molecular targets for developing strategies against viral diseases in octopus aquaculture.
(Copyright © 2025 Elsevier Inc. All rights reserved.)*

*Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.*