Treffer: Identification and screening of potential RNAi targets in the bean bug Riptortus pedestris via virus-induced gene silencing and root soaking.
Title:
Identification and screening of potential RNAi targets in the bean bug Riptortus pedestris via virus-induced gene silencing and root soaking.
Authors:
Chen ZX; Anhui Engineering Research Center for Green Production Technology of Drought Grain Crops, College of Life Sciences, Huaibei Normal University, Huaibei, China.; Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, College of Life Sciences, Huaibei Normal University, Huaibei, China.; Huaibei City Key Laboratory of Green Prevention and Control of Crop Diseases and Pests, College of Life Sciences, Huaibei Normal University, Huaibei, China., Xuan JL; Anhui Engineering Research Center for Green Production Technology of Drought Grain Crops, College of Life Sciences, Huaibei Normal University, Huaibei, China.; Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, College of Life Sciences, Huaibei Normal University, Huaibei, China.; Huaibei City Key Laboratory of Green Prevention and Control of Crop Diseases and Pests, College of Life Sciences, Huaibei Normal University, Huaibei, China., Willadsen PC; Department of Ecology and Evolutionary Biology, University of Kansas, Lawrence, KS, USA., Lorenzen M; Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, USA., Li JB; Suzhou Vocational and Technical College, Suzhou, China., Liu WX; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China., Zhang YN; Anhui Engineering Research Center for Green Production Technology of Drought Grain Crops, College of Life Sciences, Huaibei Normal University, Huaibei, China.; Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, College of Life Sciences, Huaibei Normal University, Huaibei, China.; Huaibei City Key Laboratory of Green Prevention and Control of Crop Diseases and Pests, College of Life Sciences, Huaibei Normal University, Huaibei, China., Li SP; Anhui Engineering Research Center for Green Production Technology of Drought Grain Crops, College of Life Sciences, Huaibei Normal University, Huaibei, China.; Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, College of Life Sciences, Huaibei Normal University, Huaibei, China.; Huaibei City Key Laboratory of Green Prevention and Control of Crop Diseases and Pests, College of Life Sciences, Huaibei Normal University, Huaibei, China.
Source:
Pest management science [Pest Manag Sci] 2026 Feb; Vol. 82 (2), pp. 1400-1413. Date of Electronic Publication: 2025 Oct 16.
Publication Type:
Journal Article
Language:
English
Journal Info:
Publisher: Published for SCI by Wiley Country of Publication: England NLM ID: 100898744 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1526-4998 (Electronic) Linking ISSN: 1526498X NLM ISO Abbreviation: Pest Manag Sci Subsets: MEDLINE
Imprint Name(s):
Original Publication: West Sussex, UK : Published for SCI by Wiley, c2000-
MeSH Terms:
References:
Lim UT, Occurrence and control method of Riptortus pedestris (Hemiptera: Alydidae): Korean perspectives. Korean J Appl Entomol 52:437–448 (2013).
Li K, Zhang X, Guo J, Penn H, Wu T, Li L et al., Feeding of Riptortus pedestris on soybean plants, the primary cause of soybean staygreen syndrome in the Huang‐Huai‐Hai river basin. Crop J 7:360–367 (2019).
Zhang H, Wang Y, Wang Z, Ding W, Xu K, Li L et al., Modelling the current and future potential distribution of the bean bug Riptortus pedestris with increasingly serious damage to soybean. Pest Manag Sci 78:4340–4352 (2022).
Park SB, Koo HN, Seok SJ, Kim HK, Yi HJ and Kim GH, Feeding behavior comparison of bean bugs, Riptortus pedestris and Halyomorpha halys on different soybean cultivars. Insects 14:322–333 (2023).
Alim MA and Taek LU, Refrigerated eggs of Riptortus pedestris (Hemiptera: Alydidae) added to aggregation pheromone traps increase field parasitism in soybean. J Econ Entomol 104:1833–1839 (2011).
Takeuchi H and Endo N, Insecticide susceptibility of Nezara viridula (Heteroptera: Pentatomidae) and three other stink bug species composing a soybean pest complex in Japan. J Econ Entomol 105:1024–1033 (2012).
Vogel E, Santos D, Mingels L, Verdonckt TW and Broeck JV, RNA interference in insects: protecting beneficials and controlling pests. Front Physiol 9:1912–1933 (2019).
Li SP, Chen ZX, Gao G, Bao YQ, Fang WY, Zhang YN et al., Development of an agroinfiltration‐based transient hairpin RNA expression system in pak choi leaves (Brassica rapa ssp. chinensis) for RNA interference against Liriomyza sativae. Pestic Biochem Physiol 204:106091–106099 (2024).
Kim DH and Rossi JJ, RNAi mechanisms and applications. BioTechniques 44:613–616 (2008).
Silver K, Cooper AM and Zhu KY, Strategies for enhancing the efficiency of RNA interference in insects. Pest Manag Sci 77:2645–2658 (2021).
Lu Y, Deng X, Zhu Q, Wu D, Zhong J, Wen L et al., The dsRNA delivery, targeting and application in pest control. Agronomy 13:714–729 (2023).
Ikeno S, Qian J, Cai C, Ma Z, Li J, Yin M et al., Spray method application of transdermal dsRNA delivery system for efficient gene silencing and pest control on soybean aphid Aphis glycines. J Pest Sci 93:449–459 (2020).
Joga MR, Zotti MJ, Smagghe G and Christiaens O, RNAi efficiency, systemic properties, and novel delivery methods for pest insect control: what we know so far. Front Physiol 7:553–567 (2016).
Li H, Guan R, Guo H and Miao X, New insights into an RNAi approach for plant defence against piercing‐sucking and stem‐borer insect pests. Plant Cell Environ 38:2277–2285 (2015).
Caccia S, Astarita F, Barra E, Di Lelio I, Varricchio P and Pennacchio F, Enhancement of bacillus thuringiensis toxicity by feeding Spodoptera littoralis larvae with bacteria expressing immune suppressive dsRNA. J Pest Sci 93:303–314 (2020).
Di Lelio I, Barra E, Coppola M, Corrado G, Rao R and Caccia S, Transgenic plants expressing immunosuppressive dsRNA improve entomopathogen efficacy against Spodoptera littoralis larvae. J Pest Sci 95:1413–1428 (2022).
Bai M, Liu ZL, Zhou YY, Xu QX, Liu TX and Tian HG, Influence of diverse storage conditions of double‐stranded RNA in vitro on the RNA interference efficiency in vivo insect Tribolium castaneum. Pest Manag Sci 79:45–54 (2023).
Liu F, Yang B, Zhang A, Ding D and Wang G, Plant‐mediated RNAi for controlling Apolygus lucorum. Front Plant Sci 10:64–74 (2019).
Gong C, Yang Z, Hu Y, Wu Q, Wang S, Guo Z et al., Silencing of the BtTPS genes by transgenic plant‐mediated RNAi to control Bemisia tabaci MED. Pest Manag Sci 78:1128–1137 (2022).
Senthil‐Kumar M and Mysore KS, Tobacco rattle virus‐based virus‐induced gene silencing in Nicotiana benthamiana. Nat Protoc 9:1549–1562 (2014).
Yang Z, Guo Z, Gong C, Xia J, Hu Y, Zhong J et al., Two horizontally acquired bacterial genes steer the exceptionally efficient and flexible nitrogenous waste cycling in whiteflies. Sci Adv 10:1–20 (2024).
Rossi M, Ottati S, Bucci L, Fusco A, Abbà S, Bosco D et al., Lab‐scale method for plant‐mediated delivery of dsRNAs to phloem‐feeding leafhoppers. J Pest Sci 97:455–467 (2024).
Siddique AB, Rahman MZ, Gain N, Rahman MS and Rahman J, Harnessing double‐stranded RNA (dsRNA): a sustainable approach to pest management. Physiol Mol Biol Plants 31:1237–1257 (2025). https://doi.org/10.1007/s12298-025-01564-8.
Spit J, Philips A, Wynant N, Santos D, Plaetinck G and Vanden Broeck J, Knockdown of nuclease activity in the gut enhances RNAi efficiency in the Colorado potato beetle, Leptinotarsa decemlineata, but not in the desert locust, Schistocerca gregaria. Insect Biochem Mol Biol 81:103–116 (2017).
Rössner C, Lotz D and Becker A, VIGS goes viral: how VIGS transforms our understanding of plant science. Annu Rev Plant Biol 73:703–728 (2022).
Shi B, He H, Zhao C, Lei C, Li J and Yan FM, Potential of virus‐mediated RNAi of insect genes in plants to control aphids. J Agric Food Chem 73:7716–7724 (2025). https://doi.org/10.1021/acs.jafc.4c09681.
Zhang J, Tian J, Tai DQ, Li KT, Zhu YJ and Yao YC, An optimized TRV‐based virus‐induced gene silencing protocol for malus crabapple. Plant Cell Tissue Organ Cult 126:499–509 (2016).
Xu Y, Cui Y, Chen H, Pu Y, Zhang C and Huang H, Development and application of the TRV‐induced gene‐silencing system in different rhododendron species. Plant Cell Tissue Organ Cult 157:61–77 (2024).
Xiang H, Li MW, Guo JH, Jiang JH and Huang YP, Influence of RNAi knockdown for E‐complex genes on the silkworm proleg development. Arch Insect Biochem Physiol 76:1–11 (2011).
Wu JJ, Mu LL, Kang WN, Ze LJ, Shen CH, Jin L et al., RNA interference targeting ecdysone receptor blocks the larval‐pupal transition in Henosepilachna vigintioctopunctata. Insect sci 28:419–429 (2021).
Chen D, Yang X, Yang D, Liu Y, Wang Y, Luo X et al., The RNase III enzyme Dicer1 is essential for larval development in Bombyx mori. Insect Sci 30:1309–1324 (2023).
Jiao Y, Zhu GH, Chen X, Dhandapani RK and Palli SR, The function of mitochondrial regulator LRPPRC in Aedes aegypti and its potential for mosquito control. J Pest Sci 97:1045–1057 (2024).
Tamura K, Stecher G and Kumar S, MEGA11: molecular evolutionary genetics analysis version 11. Mol Biol Evol 38:3022–3027 (2021).
Chen C, Wu Y, Li J, Wang X, Zeng Z, Xu J et al., TBtools‐II: a “one for all, all for one” bioinformatics platform for biological big‐data mining. Mol Plant 16:1733–1742 (2023).
Livak KJ and Schmittgen TD, Analysis of relative gene expression data using real‐time quantitative PCR and the 2−ΔΔCT method. Methods 25:402–408 (2001).
Wu C, Jia L and Goggin F, The reliability of virus‐induced gene silencing experiments using tobacco rattle virus in tomato is influenced by the size of the vector control. Mol Plant Pathol 12:299–305 (2011).
Ma YF, Zhao YQ, Zhou YY, Feng HY, Gong LL, Zhang MQ et al., Nanoparticle‐delivered RNAi‐based pesticide target screening for the rice pest white‐backed planthopper and risk assessment for a natural predator. Sci Total Environ 926:171286 (2024).
Antiabong JF, Ngoepe MG and Abechi AS, Semi‐quantitative digital analysis of polymerase chain reaction‐electrophoresis gel: potential applications in low‐income veterinary laboratories. Vet World 9:935–940 (2016).
Tomlinson C, Rajasekaran A, Brochu‐Gaudreau K, Dubois C, Farmilo AJ, Gris P et al., A convenient analytic method for gel quantification using ImageJ paired with python or R. PLoS One 19:1–18 (2024).
Cagliari D, Dias NP, Galdeano DM, Dos Santos EÁ, Smagghe G and Zotti MJ, Management of pest insects and plant diseases by non‐transformative RNAi. Front Plant Sci 10:1319–1337 (2019).
de Andrade EC and Hunter WB, RNA interference‐natural gene‐based technology for highly specific pest control (HiSPeC), in RNA Interference. IntechOpen, London, United Kingdom (2016). https://doi.org/10.5772/61612.
Ikeno T, Ishikawa K, Numata H and Goto SG, Circadian clock gene clock is involved in the photoperiodic response of the bean bug Riptortus pedestris. Physiol Entomol 38:157–162 (2013).
Wang H, Ying J, Mao Z, Wang B, Ye Z, Chen Y et al., Identification and functional analysis of the female determiner gene in the bean bug, Riptortus pedestris. Pest Manag Sci 80:1240–1248 (2024).
Feng H and Jander G, Rapid screening of Myzus persicae (green peach aphid) RNAi targets using tobacco rattle virus, in RNAi Strategies for Pest Management: Methods and Protocols. Springer US, New York, NY, pp. 105–117 (2021).
Feng H, Chen W, Hussain S, Shakir S, Tzin V, Adegbayi F et al., Horizontally transferred genes as RNA interference targets for aphid and whitefly control. Plant Biotechnol J 21:754–768 (2023).
Kolliopoulou A, Taning CN, Smagghe G and Swevers L, Viral delivery of dsRNA for control of insect agricultural pests and vectors of human disease: prospects and challenges. Front Physiol 8:399–423 (2017).
Ghosh SKB, Hunter WB, Park AL and Gundersen‐Rindal DE, Double strand RNA delivery system for plant‐sap‐feeding insects. PLoS One 12:1–19 (2017).
Majidiani S, PourAbad RF, Laudani F, Campolo O, Zappalà L, Rahmani S et al., RNAi in Tuta absoluta management: effects of injection and root delivery of dsRNAs. J Pest Sci 92:1409–1419 (2019).
Casanova J, Sanchez‐Herrero E, Busturia A and Morata G, Double and triple mutant combinations of bithorax complex of drosophila. EMBO J 6:3103–3109 (1987).
Lewis DL, DeCamillis M and Bennett RL, Distinct roles of the homeotic genes Ubx and abd‐a in beetle embryonic abdominal appendage development. Proc Natl Acad Sci 97:4504–4509 (2000).
Singh NP and Mishra RK, Role of abd‐a and Abd‐B in development of abdominal epithelia breaks posterior prevalence rule. PLoS Genet 10:1–13 (2014).
Chen SJ, Liu XY, Zhang JL, Yang ZN and Xu HJ, Abdominal‐B contributes to abdominal identity in the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae). Insect Mol Biol 31:447–456 (2022).
Djuranovic S, Nahvi A and Green R, miRNA‐mediated gene silencing by translational repression followed by mRNA deadenylation and decay. Science 336:237–240 (2012).
Liu Z, Xu J, Ling L, Luo X, Yang D, Yang X et al., miR‐34 regulates larval growth and wing morphogenesis by directly modulating ecdysone signalling and cuticle protein in Bombyx mori. RNA Biol 17:1342–1351 (2020).
Liu Z, Ling L, Xu J, Zeng B, Huang Y, Shang P et al., MicroRNA‐14 regulates larval development time in Bombyx mori. Insect Biochem Mol Biol 93:57–65 (2018).
He K, Xiao H, Sun Y, Situ G, Xi Y and Li F, microRNA‐14 as an efficient suppressor to switch off ecdysone production after ecdysis in insects. RNA Biol 16:1313–1325 (2019).
Gomez‐Orte E and Belles X, MicroRNA‐dependent metamorphosis in hemimetabolan insects. Proc Natl Acad Sci 106:21678–21682 (2009).
Wang YL, Yang ML, Jiang F, Zhang JZ and Kang L, MicroRNA‐dependent development revealed by RNA interference‐mediated gene silencing of LmDicer1 in the migratory locust. Insect Sci 20:53–60 (2013).
Koelle MR, Talbot WS, Segraves WA, Bender MT, Cherbas P and Hogness DS, The drosophila EcR gene encodes an ecdysone receptor, a new member of the steroid receptor superfamily. Cell 67:59–77 (1991).
Ullah F, Gul H, Tariq K, Hafeez M, Desneux N, Gao X et al., RNA interference‐mediated silencing of ecdysone receptor (EcR) gene causes lethal and sublethal effects on melon aphid. Aphis gossypii Entomol Gen 42:791–797 (2022).
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Xu QY, Deng P, Zhang Q, Li A, Fu KY, Guo WC et al., Ecdysone receptor isoforms play distinct roles in larval‐pupal‐adult transition in Leptinotarsa decemlineata. Insect sci 27:487–499 (2020).
Yu J, Song H, Wang Y, Liu Z, Wang H and Xu B, 20‐hydroxyecdysone upregulates ecdysone receptor (ECR) gene to promote pupation in the honeybee, Apis mellifera Ligustica. Integr Comp Biol 63:288–303 (2023).
Debray FG, Morin C, Janvier A, Villeneuve J, Maranda B, Laframboise R et al., LRPPRC mutations cause a phenotypically distinct form of Leigh syndrome with cytochrome c oxidase deficiency. J Med Genet 48:183–189 (2011).
Jiao Y and Palli SR, Mitochondria dysfunction impairs Tribolium castaneum wing development during metamorphosis. Commun Biol 5:1252–1263 (2022).
Li K, Zhang X, Guo J, Penn H, Wu T, Li L et al., Feeding of Riptortus pedestris on soybean plants, the primary cause of soybean staygreen syndrome in the Huang‐Huai‐Hai river basin. Crop J 7:360–367 (2019).
Zhang H, Wang Y, Wang Z, Ding W, Xu K, Li L et al., Modelling the current and future potential distribution of the bean bug Riptortus pedestris with increasingly serious damage to soybean. Pest Manag Sci 78:4340–4352 (2022).
Park SB, Koo HN, Seok SJ, Kim HK, Yi HJ and Kim GH, Feeding behavior comparison of bean bugs, Riptortus pedestris and Halyomorpha halys on different soybean cultivars. Insects 14:322–333 (2023).
Alim MA and Taek LU, Refrigerated eggs of Riptortus pedestris (Hemiptera: Alydidae) added to aggregation pheromone traps increase field parasitism in soybean. J Econ Entomol 104:1833–1839 (2011).
Takeuchi H and Endo N, Insecticide susceptibility of Nezara viridula (Heteroptera: Pentatomidae) and three other stink bug species composing a soybean pest complex in Japan. J Econ Entomol 105:1024–1033 (2012).
Vogel E, Santos D, Mingels L, Verdonckt TW and Broeck JV, RNA interference in insects: protecting beneficials and controlling pests. Front Physiol 9:1912–1933 (2019).
Li SP, Chen ZX, Gao G, Bao YQ, Fang WY, Zhang YN et al., Development of an agroinfiltration‐based transient hairpin RNA expression system in pak choi leaves (Brassica rapa ssp. chinensis) for RNA interference against Liriomyza sativae. Pestic Biochem Physiol 204:106091–106099 (2024).
Kim DH and Rossi JJ, RNAi mechanisms and applications. BioTechniques 44:613–616 (2008).
Silver K, Cooper AM and Zhu KY, Strategies for enhancing the efficiency of RNA interference in insects. Pest Manag Sci 77:2645–2658 (2021).
Lu Y, Deng X, Zhu Q, Wu D, Zhong J, Wen L et al., The dsRNA delivery, targeting and application in pest control. Agronomy 13:714–729 (2023).
Ikeno S, Qian J, Cai C, Ma Z, Li J, Yin M et al., Spray method application of transdermal dsRNA delivery system for efficient gene silencing and pest control on soybean aphid Aphis glycines. J Pest Sci 93:449–459 (2020).
Joga MR, Zotti MJ, Smagghe G and Christiaens O, RNAi efficiency, systemic properties, and novel delivery methods for pest insect control: what we know so far. Front Physiol 7:553–567 (2016).
Li H, Guan R, Guo H and Miao X, New insights into an RNAi approach for plant defence against piercing‐sucking and stem‐borer insect pests. Plant Cell Environ 38:2277–2285 (2015).
Caccia S, Astarita F, Barra E, Di Lelio I, Varricchio P and Pennacchio F, Enhancement of bacillus thuringiensis toxicity by feeding Spodoptera littoralis larvae with bacteria expressing immune suppressive dsRNA. J Pest Sci 93:303–314 (2020).
Di Lelio I, Barra E, Coppola M, Corrado G, Rao R and Caccia S, Transgenic plants expressing immunosuppressive dsRNA improve entomopathogen efficacy against Spodoptera littoralis larvae. J Pest Sci 95:1413–1428 (2022).
Bai M, Liu ZL, Zhou YY, Xu QX, Liu TX and Tian HG, Influence of diverse storage conditions of double‐stranded RNA in vitro on the RNA interference efficiency in vivo insect Tribolium castaneum. Pest Manag Sci 79:45–54 (2023).
Liu F, Yang B, Zhang A, Ding D and Wang G, Plant‐mediated RNAi for controlling Apolygus lucorum. Front Plant Sci 10:64–74 (2019).
Gong C, Yang Z, Hu Y, Wu Q, Wang S, Guo Z et al., Silencing of the BtTPS genes by transgenic plant‐mediated RNAi to control Bemisia tabaci MED. Pest Manag Sci 78:1128–1137 (2022).
Senthil‐Kumar M and Mysore KS, Tobacco rattle virus‐based virus‐induced gene silencing in Nicotiana benthamiana. Nat Protoc 9:1549–1562 (2014).
Yang Z, Guo Z, Gong C, Xia J, Hu Y, Zhong J et al., Two horizontally acquired bacterial genes steer the exceptionally efficient and flexible nitrogenous waste cycling in whiteflies. Sci Adv 10:1–20 (2024).
Rossi M, Ottati S, Bucci L, Fusco A, Abbà S, Bosco D et al., Lab‐scale method for plant‐mediated delivery of dsRNAs to phloem‐feeding leafhoppers. J Pest Sci 97:455–467 (2024).
Siddique AB, Rahman MZ, Gain N, Rahman MS and Rahman J, Harnessing double‐stranded RNA (dsRNA): a sustainable approach to pest management. Physiol Mol Biol Plants 31:1237–1257 (2025). https://doi.org/10.1007/s12298-025-01564-8.
Spit J, Philips A, Wynant N, Santos D, Plaetinck G and Vanden Broeck J, Knockdown of nuclease activity in the gut enhances RNAi efficiency in the Colorado potato beetle, Leptinotarsa decemlineata, but not in the desert locust, Schistocerca gregaria. Insect Biochem Mol Biol 81:103–116 (2017).
Rössner C, Lotz D and Becker A, VIGS goes viral: how VIGS transforms our understanding of plant science. Annu Rev Plant Biol 73:703–728 (2022).
Shi B, He H, Zhao C, Lei C, Li J and Yan FM, Potential of virus‐mediated RNAi of insect genes in plants to control aphids. J Agric Food Chem 73:7716–7724 (2025). https://doi.org/10.1021/acs.jafc.4c09681.
Zhang J, Tian J, Tai DQ, Li KT, Zhu YJ and Yao YC, An optimized TRV‐based virus‐induced gene silencing protocol for malus crabapple. Plant Cell Tissue Organ Cult 126:499–509 (2016).
Xu Y, Cui Y, Chen H, Pu Y, Zhang C and Huang H, Development and application of the TRV‐induced gene‐silencing system in different rhododendron species. Plant Cell Tissue Organ Cult 157:61–77 (2024).
Xiang H, Li MW, Guo JH, Jiang JH and Huang YP, Influence of RNAi knockdown for E‐complex genes on the silkworm proleg development. Arch Insect Biochem Physiol 76:1–11 (2011).
Wu JJ, Mu LL, Kang WN, Ze LJ, Shen CH, Jin L et al., RNA interference targeting ecdysone receptor blocks the larval‐pupal transition in Henosepilachna vigintioctopunctata. Insect sci 28:419–429 (2021).
Chen D, Yang X, Yang D, Liu Y, Wang Y, Luo X et al., The RNase III enzyme Dicer1 is essential for larval development in Bombyx mori. Insect Sci 30:1309–1324 (2023).
Jiao Y, Zhu GH, Chen X, Dhandapani RK and Palli SR, The function of mitochondrial regulator LRPPRC in Aedes aegypti and its potential for mosquito control. J Pest Sci 97:1045–1057 (2024).
Tamura K, Stecher G and Kumar S, MEGA11: molecular evolutionary genetics analysis version 11. Mol Biol Evol 38:3022–3027 (2021).
Chen C, Wu Y, Li J, Wang X, Zeng Z, Xu J et al., TBtools‐II: a “one for all, all for one” bioinformatics platform for biological big‐data mining. Mol Plant 16:1733–1742 (2023).
Livak KJ and Schmittgen TD, Analysis of relative gene expression data using real‐time quantitative PCR and the 2−ΔΔCT method. Methods 25:402–408 (2001).
Wu C, Jia L and Goggin F, The reliability of virus‐induced gene silencing experiments using tobacco rattle virus in tomato is influenced by the size of the vector control. Mol Plant Pathol 12:299–305 (2011).
Ma YF, Zhao YQ, Zhou YY, Feng HY, Gong LL, Zhang MQ et al., Nanoparticle‐delivered RNAi‐based pesticide target screening for the rice pest white‐backed planthopper and risk assessment for a natural predator. Sci Total Environ 926:171286 (2024).
Antiabong JF, Ngoepe MG and Abechi AS, Semi‐quantitative digital analysis of polymerase chain reaction‐electrophoresis gel: potential applications in low‐income veterinary laboratories. Vet World 9:935–940 (2016).
Tomlinson C, Rajasekaran A, Brochu‐Gaudreau K, Dubois C, Farmilo AJ, Gris P et al., A convenient analytic method for gel quantification using ImageJ paired with python or R. PLoS One 19:1–18 (2024).
Cagliari D, Dias NP, Galdeano DM, Dos Santos EÁ, Smagghe G and Zotti MJ, Management of pest insects and plant diseases by non‐transformative RNAi. Front Plant Sci 10:1319–1337 (2019).
de Andrade EC and Hunter WB, RNA interference‐natural gene‐based technology for highly specific pest control (HiSPeC), in RNA Interference. IntechOpen, London, United Kingdom (2016). https://doi.org/10.5772/61612.
Ikeno T, Ishikawa K, Numata H and Goto SG, Circadian clock gene clock is involved in the photoperiodic response of the bean bug Riptortus pedestris. Physiol Entomol 38:157–162 (2013).
Wang H, Ying J, Mao Z, Wang B, Ye Z, Chen Y et al., Identification and functional analysis of the female determiner gene in the bean bug, Riptortus pedestris. Pest Manag Sci 80:1240–1248 (2024).
Feng H and Jander G, Rapid screening of Myzus persicae (green peach aphid) RNAi targets using tobacco rattle virus, in RNAi Strategies for Pest Management: Methods and Protocols. Springer US, New York, NY, pp. 105–117 (2021).
Feng H, Chen W, Hussain S, Shakir S, Tzin V, Adegbayi F et al., Horizontally transferred genes as RNA interference targets for aphid and whitefly control. Plant Biotechnol J 21:754–768 (2023).
Kolliopoulou A, Taning CN, Smagghe G and Swevers L, Viral delivery of dsRNA for control of insect agricultural pests and vectors of human disease: prospects and challenges. Front Physiol 8:399–423 (2017).
Ghosh SKB, Hunter WB, Park AL and Gundersen‐Rindal DE, Double strand RNA delivery system for plant‐sap‐feeding insects. PLoS One 12:1–19 (2017).
Majidiani S, PourAbad RF, Laudani F, Campolo O, Zappalà L, Rahmani S et al., RNAi in Tuta absoluta management: effects of injection and root delivery of dsRNAs. J Pest Sci 92:1409–1419 (2019).
Casanova J, Sanchez‐Herrero E, Busturia A and Morata G, Double and triple mutant combinations of bithorax complex of drosophila. EMBO J 6:3103–3109 (1987).
Lewis DL, DeCamillis M and Bennett RL, Distinct roles of the homeotic genes Ubx and abd‐a in beetle embryonic abdominal appendage development. Proc Natl Acad Sci 97:4504–4509 (2000).
Singh NP and Mishra RK, Role of abd‐a and Abd‐B in development of abdominal epithelia breaks posterior prevalence rule. PLoS Genet 10:1–13 (2014).
Chen SJ, Liu XY, Zhang JL, Yang ZN and Xu HJ, Abdominal‐B contributes to abdominal identity in the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae). Insect Mol Biol 31:447–456 (2022).
Djuranovic S, Nahvi A and Green R, miRNA‐mediated gene silencing by translational repression followed by mRNA deadenylation and decay. Science 336:237–240 (2012).
Liu Z, Xu J, Ling L, Luo X, Yang D, Yang X et al., miR‐34 regulates larval growth and wing morphogenesis by directly modulating ecdysone signalling and cuticle protein in Bombyx mori. RNA Biol 17:1342–1351 (2020).
Liu Z, Ling L, Xu J, Zeng B, Huang Y, Shang P et al., MicroRNA‐14 regulates larval development time in Bombyx mori. Insect Biochem Mol Biol 93:57–65 (2018).
He K, Xiao H, Sun Y, Situ G, Xi Y and Li F, microRNA‐14 as an efficient suppressor to switch off ecdysone production after ecdysis in insects. RNA Biol 16:1313–1325 (2019).
Gomez‐Orte E and Belles X, MicroRNA‐dependent metamorphosis in hemimetabolan insects. Proc Natl Acad Sci 106:21678–21682 (2009).
Wang YL, Yang ML, Jiang F, Zhang JZ and Kang L, MicroRNA‐dependent development revealed by RNA interference‐mediated gene silencing of LmDicer1 in the migratory locust. Insect Sci 20:53–60 (2013).
Koelle MR, Talbot WS, Segraves WA, Bender MT, Cherbas P and Hogness DS, The drosophila EcR gene encodes an ecdysone receptor, a new member of the steroid receptor superfamily. Cell 67:59–77 (1991).
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Grant Information:
03106233 Doctoral Research Initiation Foundation Program at Huaibei Normal University; 03106237 Doctoral Research Initiation Foundation Program at Huaibei Normal University; 2023AH020040 Anhui Province University Natural Science Research Outstanding Youth Project; 2023AH040365 Natural Science Fund of Education Department of Anhui Province, China
Contributed Indexing:
Keywords: RNA interference; Riptortus pedestris; pest control; root soaking; tobacco rattle virus‐induced gene silencing system
Substance Nomenclature:
0 (RNA, Double-Stranded)
SCR Organism:
Tobacco rattle virus
Entry Date(s):
Date Created: 20251016 Date Completed: 20260111 Latest Revision: 20260111
Update Code:
20260130
DOI:
10.1002/ps.70289
PMID:
41099218
Database:
MEDLINE
Weitere Informationen
Background: RNA interference (RNAi) is a promising approach for the development of reliable and sustainable techniques for the control of insect pest of agricultural importance. However, gene selection and efficient double-stranded RNA (dsRNA) delivery remain major challenges in the application of RNAi technology for pest management.
Results: In this study, five potential target genes (RpAbd-A, RpAbd-B, RpDicer, RpEcR, and RpLRPPRC) were identified from the genome of Riptortus pedestris. When all target genes were individually silenced using tobacco rattle virus-induced gene silencing (TRV-VIGS) system in Nicotiana tabacum, the expression levels of messenger RNA (mRNA) transcripts in nymphs were significantly reduced by 63.5-96.8% associated with 1.8- to 4.3-fold increases in mortality rates. However, silencing Ecdysone receptor (EcR) gene caused the highest nymph mortality rate (76.7%) compared to other RNAi treatments. Subsequently, EcR gene in R. pedestris nymphs was silenced via root soaking in soybean seedlings, resulting in a 65.8% decrease of EcR expression and a 3.8-fold increase in mortality rate.
Conclusion: Two dsRNA delivery approaches, TRV-VIGS system and root soaking, were developed in R. pedestris, and gene EcR was considered as the best performing RNAi target. Our findings are of great importance to the future development of effective RNAi-based approaches to control sap-sucking pests. © 2025 Society of Chemical Industry.
(© 2025 Society of Chemical Industry.)