Treffer: Insects as Experimental Models to Study the Toxicological Effects of Nanoparticles: Concerns at the Behavioral, Physiological, Cellular, and Molecular Levels.
Title:
Insects as Experimental Models to Study the Toxicological Effects of Nanoparticles: Concerns at the Behavioral, Physiological, Cellular, and Molecular Levels.
Authors:
Monica K; Unit of Molecular Entomology, Department of Zoology, University of Madras, Guindy Campus, Chennai, India., Janarthanan S; Unit of Molecular Entomology, Department of Zoology, University of Madras, Guindy Campus, Chennai, India.
Source:
Journal of applied toxicology : JAT [J Appl Toxicol] 2026 Feb; Vol. 46 (2), pp. 419-433. Date of Electronic Publication: 2025 Oct 05.
Publication Type:
Journal Article; Review
Language:
English
Journal Info:
Publisher: John Wiley And Sons Country of Publication: England NLM ID: 8109495 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1099-1263 (Electronic) Linking ISSN: 0260437X NLM ISO Abbreviation: J Appl Toxicol Subsets: MEDLINE
Imprint Name(s):
Publication: : Chichester : John Wiley And Sons
Original Publication: [Philadelphia, Pa. : Heyden & Son, c1981-
Original Publication: [Philadelphia, Pa. : Heyden & Son, c1981-
MeSH Terms:
References:
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Alaraby, M., A. Hernández, B. Annangi, et al. 2015. “Antioxidant and Antigenotoxic Properties of CeO2 NPs and Cerium Sulphate: Studies With Drosophila melanogaster as a Promising In Vivo Model.” Nanotoxicology 9, no. 6: 749–759. https://doi.org/10.3109/17435390.2014.976284.
Anand, A. S., U. Gahlot, D. N. Prasad, and E. Kohli. 2019. “Aluminum Oxide Nanoparticles Mediated Toxicity, Loss of Appendages in Progeny of Drosophila melanogaster on Chronic Exposure.” Nanotoxicology 13, no. 7: 977–989. https://doi.org/10.1080/17435390.2019.1602680.
Andere, A. A., R. N. Platt, and D. A. Ray. 2016. “Genome Sequence of Phormia regina Meigen (Diptera: Calliphoridae): Implications for Medical, Veterinary and Forensic Research.” BMC Genomics 17: 842. https://doi.org/10.1186/s12864‐016‐3187‐z.
Armstrong, N., M. Ramamoorthy, D. Lyon, K. Jones, and A. Duttaroy. 2013. “Mechanism of Silver Nanoparticles Action on Insect Pigmentation Reveals Intervention of Copper Homeostasis.” Public Library of Science ONE 8, no. 1: e53186. https://doi.org/10.1371/journal.pone.0053186.
Arumugam, G., V. Velayutham, S. Shanmugavel, and J. Sundaram. 2016. “Efficacy of Nanostructured Silica as a Stored Pulse Protector Against the Infestation of Bruchid Beetle, Callosobruchus maculatus (Coleoptera: Bruchidae).” Applied Nanoscience 6, no. 3: 445–450. https://doi.org/10.1007/s13204‐015‐0446‐2.
Ashraf, H., and A. Qamar. 2023. “Silkworm Bombyx mori as a Model Organism: A Review.” Physiological Entomology 48, no. 4: 107–121. https://doi.org/10.1111/phen.12421.
Babczyńska, A., M. Tarnawska, K. Czaja, et al. 2024. “Adult Young as the Fragile Ontogenetic Stage of the House Crickets Dietary Exposed to GO Nanoparticles–Digestive Enzymes Perspective.” Chemosphere 367: 143641. https://doi.org/10.1016/j.chemosphere.2024.143641.
Benelli, G. 2018. “Mode of Action of Nanoparticles Against Insects.” Environmental Science and Pollution Research 25, no. 10: 1031–1037. https://doi.org/10.1007/s11356‐018‐1850‐4.
Cáceres, M., C. V. Vassena, M. D. Garcerá, and P. L. Santo‐Orihuela. 2019. “Silica Nanoparticles for Insect Pest Control.” Current Pharmceutical Design 25, no. 37: 4030–4038. https://doi.org/10.2174/1381612825666191015152855.
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Demir, E., G. Vales, B. Kaya, A. Creus, and R. Marcos. 2011. “Genotoxic Analysis of Silver Nanoparticles in Drosophila.” Nanotoxicology 5, no. 3: 417–424. https://doi.org/10.3109/17435390.2010.529176.
Demirtürk, Z., and F. Uçkan. 2025. “The Effects of Alumina and Polystyrene Nanoparticles on Global DNA Methylation, Antimicrobial Peptides and Intergenerational Inheritance of Galleria mellonella.” Drug and Chemical Toxicology 1‐14: 729–742. https://doi.org/10.1080/01480545.2025.2483970.
Demirtürk, Z., F. Uçkan, and S. Mert. 2024. “Interactions of Alumina and Polystyrene Nanoparticles With the Innate Immune System of Galleria mellonella.” Drug and Chemical Toxicology 47, no. 5: 483–495. https://doi.org/10.1080/01480545.2023.2217484.
El‐Ashram, S., A. M. Ali, S. E. Osman, S. Huang, A. M. Shouman, and D. A. Kheirallah. 2021. “Biochemical and Histological Alterations Induced by Nickel Oxide Nanoparticles in the Ground Beetle Blaps polychresta (Forskl, 1775) (Coleoptera: Tenebrionidae).” PLoS ONE 16, no. 9: e0255623. https://doi.org/10.1371/journal.pone.0255623.
Eskin, A., Ş. Öztürk, and M. Körükçü. 2019. “Determination of the Acute Toxic Effects of Zinc Oxide Nanoparticles (ZnO NPs) in Total Hemocytes Counts of Galleria mellonella (Lepidoptera: Pyralidae) With Two Different Methods.” Ecotoxicology 28: 801–808. https://doi.org/10.1007/s10646‐019‐02078‐2.
Jasrotia, P., M. Nagpal, C. M. Mishra, et al. 2022. “Nanomaterials for Postharvest Management of Insect Pests: Current State and Future Perspectives.” Frontiers in Nanotechnology 3: 811056. https://doi.org/10.3389/fnano.2021.811056.
Jiang, F., L. Liang, J. Wang, and S. Zhu. 2022. “Chromosome‐Level Genome Assembly of Bactrocera dorsalis Reveals Its Adaptation and Invasion Mechanisms.” Communications Biology 5, no. 1: 25. https://doi.org/10.1038/s42003‐021‐02966‐6.
Jovanović, B., V. J. Cvetković, and T. L. Mitrović. 2016. “Effects of Human Food Grade Titanium Dioxide Nanoparticle Dietary Exposure on Drosophila melanogaster Survival, Fecundity, Pupation and Expression of Antioxidant Genes.” Chemosphere 144: 43–49. https://doi.org/10.1016/j.chemosphere.2015.08.054.
Kah, M., S. Beulke, K. Tiede, and T. Hofmann. 2013. “Nanopesticides: State of Knowledge, Environmental Fate, and Exposure Modeling.” Critical Reviews in Environmental Science and Technology 43, no. 16: 1823–1867. https://doi.org/10.1080/10643389.2012.671750.
Karpeta‐Kaczmarek, J., M. Augustyniak, and M. Rost‐Roszkowska. 2016. “Ultrastructure of the Gut Epithelium in Acheta domesticus After Long‐Term Exposure to Nanodiamonds Supplied With Food.” Arthropod Structure & Development 45, no. 3: 253–264. https://doi.org/10.1016/j.asd.2016.02.002.
Karpeta‐Kaczmarek, J., M. Dziewięcka, M. Augustyniak, and M. Rost‐Roszkowska. 2016. “Effects of Short‐Term Exposure of Acheta domesticus to Nanodiamonds in Food: DNA Damage But no Histological Alteration in Tissues.” Carbon 110: 458–468. https://doi.org/10.1016/j.carbon.2016.09.053.
Karpeta‐Kaczmarek, J., A. Kędziorski, M. A. Augustyniak‐Jabłokow, M. Dziewięcka, and M. Augustyniak. 2018. “Chronic Toxicity of Nanodiamonds Can Disturb Development and Reproduction of Acheta domesticus L.” Environmental Research 166: 602–609. https://doi.org/10.1016/j.envres.2018.05.027.
Key, S. C. S., D. Reaves, F. Turner, and J. J. Bang. 2011. “Impacts of Silver Nanoparticle Ingestion on Pigmentation and Developmental Progression in Drosophila.” Atlas Journal of Biology 1, no. 3: 52–61. https://doi.org/10.5147/ajb.v1i3.10.
Kubo‐Irie, M., M. Shimoda, A. Sato, et al. 2015. “Effect of Nanoparticles Injected Into Larvae on Spermatogenesis in the Pupal Testis of the Sweet Potato Hornworm, Agrius convolvuli (L.).” Fundamental Toxicological Sciences 2, no. 1: 1–8. https://doi.org/10.2131/fts.2.1.
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Li, F., Z. Gu, B. Wang, et al. 2014. “Effects of the Biosynthesis and Signaling Pathway of Ecdysterone on Silkworm (Bombyx mori) Following Exposure to Titanium Dioxide Nanoparticles.” Journal of Chemical Ecology 40: 913–922. https://doi.org/10.1007/s10886‐014‐0487‐0.
Liu, X., D. Vinson, D. Abt, R. H. Hurt, and D. M. Rand. 2009. “Differential Toxicity of Carbon Nanomaterials in Drosophila: Larval Dietary Uptake Is Benign, But Adult Exposure Causes Locomotor Impairment and Mortality.” Environmental Science & Technology 43, no. 16: 6357–6363. https://doi.org/10.1021/es901079z.
Mir, A. H., A. Qamar, I. Qadir, A. H. Naqvi, and R. Begum. 2020. “Accumulation and Trafficking of Zinc Oxide Nanoparticles in an Invertebrate Model, Bombyx mori, With Insights on Their Effects on Immuno‐Competent Cells.” Scientific Reports 10, no. 1: 1617. https://doi.org/10.1038/s41598‐020‐58526‐1.
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Ni, M., F. Li, B. Wang, et al. 2015. “Effect of TiO2 Nanoparticles on the Reproduction of Silkworm.” Biological Trace Element Research 164: 106–113. https://doi.org/10.1007/s12011‐014‐0195‐1.
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Ahamed, M., M. Karns, M. Goodson, et al. 2010. “DNA Damage Response to Different Surface Chemistry of Silver Nanoparticles in Mammalian Cells.” Toxicology and Applied Pharmacology 233, no. 3: 404–410. https://doi.org/10.1016/j.taap.2008.09.015.
Alaraby, M., A. Hernández, B. Annangi, et al. 2015. “Antioxidant and Antigenotoxic Properties of CeO2 NPs and Cerium Sulphate: Studies With Drosophila melanogaster as a Promising In Vivo Model.” Nanotoxicology 9, no. 6: 749–759. https://doi.org/10.3109/17435390.2014.976284.
Anand, A. S., U. Gahlot, D. N. Prasad, and E. Kohli. 2019. “Aluminum Oxide Nanoparticles Mediated Toxicity, Loss of Appendages in Progeny of Drosophila melanogaster on Chronic Exposure.” Nanotoxicology 13, no. 7: 977–989. https://doi.org/10.1080/17435390.2019.1602680.
Andere, A. A., R. N. Platt, and D. A. Ray. 2016. “Genome Sequence of Phormia regina Meigen (Diptera: Calliphoridae): Implications for Medical, Veterinary and Forensic Research.” BMC Genomics 17: 842. https://doi.org/10.1186/s12864‐016‐3187‐z.
Armstrong, N., M. Ramamoorthy, D. Lyon, K. Jones, and A. Duttaroy. 2013. “Mechanism of Silver Nanoparticles Action on Insect Pigmentation Reveals Intervention of Copper Homeostasis.” Public Library of Science ONE 8, no. 1: e53186. https://doi.org/10.1371/journal.pone.0053186.
Arumugam, G., V. Velayutham, S. Shanmugavel, and J. Sundaram. 2016. “Efficacy of Nanostructured Silica as a Stored Pulse Protector Against the Infestation of Bruchid Beetle, Callosobruchus maculatus (Coleoptera: Bruchidae).” Applied Nanoscience 6, no. 3: 445–450. https://doi.org/10.1007/s13204‐015‐0446‐2.
Ashraf, H., and A. Qamar. 2023. “Silkworm Bombyx mori as a Model Organism: A Review.” Physiological Entomology 48, no. 4: 107–121. https://doi.org/10.1111/phen.12421.
Babczyńska, A., M. Tarnawska, K. Czaja, et al. 2024. “Adult Young as the Fragile Ontogenetic Stage of the House Crickets Dietary Exposed to GO Nanoparticles–Digestive Enzymes Perspective.” Chemosphere 367: 143641. https://doi.org/10.1016/j.chemosphere.2024.143641.
Benelli, G. 2018. “Mode of Action of Nanoparticles Against Insects.” Environmental Science and Pollution Research 25, no. 10: 1031–1037. https://doi.org/10.1007/s11356‐018‐1850‐4.
Cáceres, M., C. V. Vassena, M. D. Garcerá, and P. L. Santo‐Orihuela. 2019. “Silica Nanoparticles for Insect Pest Control.” Current Pharmceutical Design 25, no. 37: 4030–4038. https://doi.org/10.2174/1381612825666191015152855.
Cook, S. M., and J. D. McArthur. 2013. “Developing Galleria mellonella as a Model Host for Human Pathogens.” Virulence 4, no. 5: 350–353. https://doi.org/10.4161/viru.25240.
Demir, E., G. Vales, B. Kaya, A. Creus, and R. Marcos. 2011. “Genotoxic Analysis of Silver Nanoparticles in Drosophila.” Nanotoxicology 5, no. 3: 417–424. https://doi.org/10.3109/17435390.2010.529176.
Demirtürk, Z., and F. Uçkan. 2025. “The Effects of Alumina and Polystyrene Nanoparticles on Global DNA Methylation, Antimicrobial Peptides and Intergenerational Inheritance of Galleria mellonella.” Drug and Chemical Toxicology 1‐14: 729–742. https://doi.org/10.1080/01480545.2025.2483970.
Demirtürk, Z., F. Uçkan, and S. Mert. 2024. “Interactions of Alumina and Polystyrene Nanoparticles With the Innate Immune System of Galleria mellonella.” Drug and Chemical Toxicology 47, no. 5: 483–495. https://doi.org/10.1080/01480545.2023.2217484.
El‐Ashram, S., A. M. Ali, S. E. Osman, S. Huang, A. M. Shouman, and D. A. Kheirallah. 2021. “Biochemical and Histological Alterations Induced by Nickel Oxide Nanoparticles in the Ground Beetle Blaps polychresta (Forskl, 1775) (Coleoptera: Tenebrionidae).” PLoS ONE 16, no. 9: e0255623. https://doi.org/10.1371/journal.pone.0255623.
Eskin, A., Ş. Öztürk, and M. Körükçü. 2019. “Determination of the Acute Toxic Effects of Zinc Oxide Nanoparticles (ZnO NPs) in Total Hemocytes Counts of Galleria mellonella (Lepidoptera: Pyralidae) With Two Different Methods.” Ecotoxicology 28: 801–808. https://doi.org/10.1007/s10646‐019‐02078‐2.
Jasrotia, P., M. Nagpal, C. M. Mishra, et al. 2022. “Nanomaterials for Postharvest Management of Insect Pests: Current State and Future Perspectives.” Frontiers in Nanotechnology 3: 811056. https://doi.org/10.3389/fnano.2021.811056.
Jiang, F., L. Liang, J. Wang, and S. Zhu. 2022. “Chromosome‐Level Genome Assembly of Bactrocera dorsalis Reveals Its Adaptation and Invasion Mechanisms.” Communications Biology 5, no. 1: 25. https://doi.org/10.1038/s42003‐021‐02966‐6.
Jovanović, B., V. J. Cvetković, and T. L. Mitrović. 2016. “Effects of Human Food Grade Titanium Dioxide Nanoparticle Dietary Exposure on Drosophila melanogaster Survival, Fecundity, Pupation and Expression of Antioxidant Genes.” Chemosphere 144: 43–49. https://doi.org/10.1016/j.chemosphere.2015.08.054.
Kah, M., S. Beulke, K. Tiede, and T. Hofmann. 2013. “Nanopesticides: State of Knowledge, Environmental Fate, and Exposure Modeling.” Critical Reviews in Environmental Science and Technology 43, no. 16: 1823–1867. https://doi.org/10.1080/10643389.2012.671750.
Karpeta‐Kaczmarek, J., M. Augustyniak, and M. Rost‐Roszkowska. 2016. “Ultrastructure of the Gut Epithelium in Acheta domesticus After Long‐Term Exposure to Nanodiamonds Supplied With Food.” Arthropod Structure & Development 45, no. 3: 253–264. https://doi.org/10.1016/j.asd.2016.02.002.
Karpeta‐Kaczmarek, J., M. Dziewięcka, M. Augustyniak, and M. Rost‐Roszkowska. 2016. “Effects of Short‐Term Exposure of Acheta domesticus to Nanodiamonds in Food: DNA Damage But no Histological Alteration in Tissues.” Carbon 110: 458–468. https://doi.org/10.1016/j.carbon.2016.09.053.
Karpeta‐Kaczmarek, J., A. Kędziorski, M. A. Augustyniak‐Jabłokow, M. Dziewięcka, and M. Augustyniak. 2018. “Chronic Toxicity of Nanodiamonds Can Disturb Development and Reproduction of Acheta domesticus L.” Environmental Research 166: 602–609. https://doi.org/10.1016/j.envres.2018.05.027.
Key, S. C. S., D. Reaves, F. Turner, and J. J. Bang. 2011. “Impacts of Silver Nanoparticle Ingestion on Pigmentation and Developmental Progression in Drosophila.” Atlas Journal of Biology 1, no. 3: 52–61. https://doi.org/10.5147/ajb.v1i3.10.
Kubo‐Irie, M., M. Shimoda, A. Sato, et al. 2015. “Effect of Nanoparticles Injected Into Larvae on Spermatogenesis in the Pupal Testis of the Sweet Potato Hornworm, Agrius convolvuli (L.).” Fundamental Toxicological Sciences 2, no. 1: 1–8. https://doi.org/10.2131/fts.2.1.
Kumar, H., M. Panigrahi, S. Chhotaray, et al. 2018. “Red flour beetle (Tribolium castaneum): From Population Genetics to Functional Genomics.” Veterinary World 11, no. 8: 1043–1046. https://doi.org/10.14202/vetworld.2018.1043‐1046.
Li, F., Z. Gu, B. Wang, et al. 2014. “Effects of the Biosynthesis and Signaling Pathway of Ecdysterone on Silkworm (Bombyx mori) Following Exposure to Titanium Dioxide Nanoparticles.” Journal of Chemical Ecology 40: 913–922. https://doi.org/10.1007/s10886‐014‐0487‐0.
Liu, X., D. Vinson, D. Abt, R. H. Hurt, and D. M. Rand. 2009. “Differential Toxicity of Carbon Nanomaterials in Drosophila: Larval Dietary Uptake Is Benign, But Adult Exposure Causes Locomotor Impairment and Mortality.” Environmental Science & Technology 43, no. 16: 6357–6363. https://doi.org/10.1021/es901079z.
Mir, A. H., A. Qamar, I. Qadir, A. H. Naqvi, and R. Begum. 2020. “Accumulation and Trafficking of Zinc Oxide Nanoparticles in an Invertebrate Model, Bombyx mori, With Insights on Their Effects on Immuno‐Competent Cells.” Scientific Reports 10, no. 1: 1617. https://doi.org/10.1038/s41598‐020‐58526‐1.
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Contributed Indexing:
Keywords: insects; model organism; nanoparticles; review; toxicity
Entry Date(s):
Date Created: 20251006 Date Completed: 20260111 Latest Revision: 20260111
Update Code:
20260130
DOI:
10.1002/jat.4960
PMID:
41047552
Database:
MEDLINE
Weitere Informationen
In recent times, nanotechnology has evolved as a fast-developing area with possible applications in numerous fields. There is a growing concern about the potential toxicity of nanoproducts to the environment and living organisms after considering the possible utilization of various kinds of nanomaterials in diverse areas of application. Subsequently, the toxicity of nanoparticles has been studied by employing several animal models to generate data to judge the judicious use of such valuable nanomaterials. Among various animal model systems used to investigate the toxicity of nanoparticles, insects are considered valuable models due to their well-known genetics, shorter lifespan, sensitivity to environmental changes, etc. Most importantly, some of the insects as test models (e.g., Drosophila melanogaster) could be very useful in predicting human toxicity, and therefore, it may be an opportunity to reduce the use of mammalian models. This review is intended to provide a comprehensive summary of the toxicity of nanoparticles in insect systems at the behavioral, morphological, physiological, and molecular levels. The overall analysis of the general literature survey made through this review showed the vulnerability of insects to the exposure of nanoparticles, emphasizing the need for comprehensive risk assessment strategies.
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