• Single-cell RNA transcriptome...

*Result*: Single-cell RNA transcriptome analysis reveals regulatory programs of RNA binding proteins in diabetic foot ulcers.

Title:
Single-cell RNA transcriptome analysis reveals regulatory programs of RNA binding proteins in diabetic foot ulcers.
Authors:
Hu Q; Department of Endocrinology, The First Affiliated Hospital of Anhui Medical University, 218 Jixi Road, Hefei, 230022, Anhui, People's Republic of China., Liu L; Department of Endocrinology, The First Affiliated Hospital of Anhui Medical University, 218 Jixi Road, Hefei, 230022, Anhui, People's Republic of China.; Institute of Endocrinology and Metabolism, Anhui Medical University, Hefei City, 230032, Anhui Province, People's Republic of China., Pei J; Department of Endocrinology, The First Affiliated Hospital of Anhui Medical University, 218 Jixi Road, Hefei, 230022, Anhui, People's Republic of China., Yan Y; Department of Endocrinology, The First Affiliated Hospital of Anhui Medical University, 218 Jixi Road, Hefei, 230022, Anhui, People's Republic of China., Chen Y; Department of Endocrinology, The First Affiliated Hospital of Anhui Medical University, 218 Jixi Road, Hefei, 230022, Anhui, People's Republic of China., Tang S; Department of Endocrinology, The First Affiliated Hospital of Anhui Medical University, 218 Jixi Road, Hefei, 230022, Anhui, People's Republic of China. tangst.healthy@163.com.; Institute of Endocrinology and Metabolism, Anhui Medical University, Hefei City, 230032, Anhui Province, People's Republic of China. tangst.healthy@163.com.
Source:
Scientific reports [Sci Rep] 2025 Aug 23; Vol. 15 (1), pp. 31059. Date of Electronic Publication: 2025 Aug 23.
Publication Type:
Journal Article
Language:
English
Journal Info:
Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
Imprint Name(s):
Original Publication: London : Nature Publishing Group, copyright 2011-
MeSH Terms:
Diabetic Foot*/genetics , Diabetic Foot*/metabolism , Diabetic Foot*/pathology , RNA-Binding Proteins*/genetics , RNA-Binding Proteins*/metabolism , Single-Cell Analysis*/methods , Transcriptome*, Wound Healing/genetics ; Leukocytes, Mononuclear/metabolism ; Humans ; Gene Expression Profiling ; Gene Expression Regulation ; Male ; Female
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Grant Information:
82270884 National Natural Science Foundation of China
Contributed Indexing:
Keywords: Diabetic foot ulcers; HSP90; RNA binding protein; Single-cell sequencing; ZFP36
Substance Nomenclature:
0 (RNA-Binding Proteins)
Entry Date(s):
Date Created: 20250823 Date Completed: 20250824 Latest Revision: 20250827
Update Code:
20260130
PubMed Central ID:
PMC12375008
DOI:
10.1038/s41598-025-16291-z
PMID:
40849582
Database:
MEDLINE

*Further Information*

*Diabetic foot ulceration poses a serious threat to the health of patients with diabetes, and its pathogenic mechanism remains unclear. In this study, we analyzed the expression profiles of genes encoding RNA-binding proteins (RBPs) in the foot and peripheral blood mononuclear cells using single-cell sequencing data from GEO database. Our analysis revealed that RBP genes were abnormally expressed in DFUs, highly cell population-specific, and can be used as signature genes for different cell clusters. Co-expression and Gene Ontology enrichment analyses showed that differentially expressed RBP genes were highly related to the wound healing process. Genes encoding heat shock proteins were associated with 'healing-related' fibroblast differentiation. Western blotting of wound tissues from normal control and diabetic patients were performed, confirming the significant upregulation of HSP90 and downregulation of ZFP36. Analysis of the combined foot samples and peripheral blood mononuclear cell samples revealed common differentially expressed RBP genes, indicating consistency between foot and blood samples. Our results demonstrate the important roles of RBP in the healing process of chronic diabetic wounds, provide clues for further research on RBP regulatory mechanisms in certain cell types, and help develop novel therapeutic treatments for DFUs.
(© 2025. The Author(s).)*

*Declarations. Competing interests: The authors declare no competing interests.*