*Result*: Cryo-light microscopy with angstrom precision deciphers structural conformations of PIEZO1 in its native state.
Title:
Cryo-light microscopy with angstrom precision deciphers structural conformations of PIEZO1 in its native state.
Authors:
Mazal H; Max Planck Institute for the Science of Light, 91058 Erlangen, Germany.; Max-Planck-Zentrum für Physik und Medizin, 91054 Erlangen, Germany., Wieser FF; Max Planck Institute for the Science of Light, 91058 Erlangen, Germany.; Max-Planck-Zentrum für Physik und Medizin, 91054 Erlangen, Germany.; Department of Physics, Friedrich-Alexander University of Erlangen-Nürnberg, 91058 Erlangen, Germany., Bollschweiler D; Max Planck Institute of Biochemistry, 82152 Planegg, Germany., Schambony A; Max Planck Institute for the Science of Light, 91058 Erlangen, Germany.; Max-Planck-Zentrum für Physik und Medizin, 91054 Erlangen, Germany.; Department of Biology, Friedrich-Alexander University of Erlangen-Nürnberg, 91058 Erlangen, Germany., Sandoghdar V; Max Planck Institute for the Science of Light, 91058 Erlangen, Germany.; Max-Planck-Zentrum für Physik und Medizin, 91054 Erlangen, Germany.; Department of Physics, Friedrich-Alexander University of Erlangen-Nürnberg, 91058 Erlangen, Germany.
Source:
Science advances [Sci Adv] 2025 Aug 22; Vol. 11 (34), pp. eadw4402. Date of Electronic Publication: 2025 Aug 20.
Publication Type:
Journal Article
Language:
English
Journal Info:
Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 101653440 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2375-2548 (Electronic) Linking ISSN: 23752548 NLM ISO Abbreviation: Sci Adv Subsets: MEDLINE
Imprint Name(s):
Original Publication: Washington, DC : American Association for the Advancement of Science, [2015]-
MeSH Terms:
References:
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Ultramicroscopy. 2015 Nov;158:26-32. (PMID: 26103047)
Biol Cell. 2011 Sep;103(9):405-20. (PMID: 21812762)
Commun Biol. 2022 May 20;5(1):487. (PMID: 35595960)
Front Immunol. 2019 Apr 05;10:675. (PMID: 31024536)
Elife. 2020 Apr 08;9:. (PMID: 32267232)
J Struct Biol. 2015 May;190(2):200-14. (PMID: 25839831)
Proc Natl Acad Sci U S A. 2022 Oct 4;119(40):e2208027119. (PMID: 36166475)
Neuron. 2025 Feb 19;113(4):590-604.e6. (PMID: 39719701)
Biochem Soc Trans. 2023 Dec 20;51(6):2041-2059. (PMID: 38015555)
Nature. 2020 Nov;587(7835):567-576. (PMID: 33239794)
Elife. 2017 Dec 12;6:. (PMID: 29231809)
Structure. 2016 Dec 6;24(12):2198-2206. (PMID: 27818102)
Nature. 2018 Feb 22;554(7693):481-486. (PMID: 29261642)
Proc Natl Acad Sci U S A. 2022 Oct 4;119(40):e2208034119. (PMID: 36166476)
J Gen Physiol. 2020 Aug 3;152(8):. (PMID: 32582958)
Q Rev Biophys. 1988 May;21(2):129-228. (PMID: 3043536)
Nature. 2000 May 18;405(6784):325-8. (PMID: 10830956)
Nature. 2019 Sep;573(7773):230-234. (PMID: 31435018)
FEBS Lett. 2020 Oct;594(20):3243-3261. (PMID: 33020915)
Nat Commun. 2019 Mar 13;10(1):1200. (PMID: 30867417)
Proc Natl Acad Sci U S A. 2020 Jun 23;117(25):13937-13944. (PMID: 32513734)
Proc Natl Acad Sci U S A. 2023 May 2;120(18):e2302325120. (PMID: 37098056)
J Gen Physiol. 2022 Jul 4;154(7):. (PMID: 35593732)
Proc Natl Acad Sci U S A. 2025 Jan 7;122(1):e2421275121. (PMID: 39739811)
iScience. 2021 Aug 08;24(9):102959. (PMID: 34466785)
J Struct Biol. 2017 May;198(2):124-133. (PMID: 28344036)
J Am Chem Soc. 2009 Jun 10;131(22):7484-5. (PMID: 19449872)
Sci Rep. 2019 Feb 4;9(1):1369. (PMID: 30718653)
Microscopy (Oxf). 2020 Dec 3;69(6):350-359. (PMID: 32447402)
Biophys J. 2016 Feb 23;110(4):758-65. (PMID: 26910419)
Nature. 2021 Aug;596(7873):583-589. (PMID: 34265844)
Nature. 2023 Aug;620(7976):1117-1125. (PMID: 37587339)
J Struct Biol. 2005 Sep;151(3):250-62. (PMID: 16125414)
Nature. 2024 Jun;630(8016):493-500. (PMID: 38718835)
Nature. 2023 May;617(7962):711-716. (PMID: 37225882)
Methods Cell Biol. 2007;79:7-21. (PMID: 17327150)
Science. 2024 Oct 11;386(6718):180-187. (PMID: 39388556)
Science. 2010 Oct 1;330(6000):55-60. (PMID: 20813920)
Nature. 2022 Apr;604(7905):377-383. (PMID: 35388220)
Elife. 2022 May 26;11:. (PMID: 35616526)
Elife. 2022 Dec 14;11:. (PMID: 36515266)
Nat Rev Mol Cell Biol. 2024 Nov;25(11):886-903. (PMID: 39251883)
Nat Struct Mol Biol. 2016 Jun;23(6):481-6. (PMID: 27273631)
J Struct Biol. 2005 Oct;152(1):36-51. (PMID: 16182563)
Nucleic Acids Res. 2018 Jan 9;46(1):1-10. (PMID: 29177436)
Biochim Biophys Acta. 1975 Mar 25;415(1):29-79. (PMID: 1091302)
Nature. 2018 Feb 22;554(7693):487-492. (PMID: 29469092)
Annu Rev Phys Chem. 2021 Apr 20;72:253-278. (PMID: 33441030)
Science. 2020 Jan 17;367(6475):. (PMID: 31949053)
Nat Methods. 2017 Feb;14(2):141-144. (PMID: 28068317)
Nature. 2019 Sep;573(7773):225-229. (PMID: 31435011)
Elife. 2015 Aug 11;4:. (PMID: 26259873)
Elife. 2018 Nov 27;7:. (PMID: 30480546)
Acta Biochim Biophys Sin (Shanghai). 2016 Sep;48(9):859-61. (PMID: 27521793)
Proc Natl Acad Sci U S A. 1975 May;72(5):1758-62. (PMID: 1057168)
Nat Commun. 2025 Jan 20;16(1):855. (PMID: 39833141)
Protein Sci. 2018 Jan;27(1):14-25. (PMID: 28710774)
Substance Nomenclature:
0 (Ion Channels)
0 (Piezo1 protein, mouse)
0 (Piezo1 protein, mouse)
Entry Date(s):
Date Created: 20250820 Date Completed: 20250820 Latest Revision: 20250823
Update Code:
20260130
PubMed Central ID:
PMC12366687
DOI:
10.1126/sciadv.adw4402
PMID:
40834076
Database:
MEDLINE
*Further Information*
*Investigations based on cryo-electron microscopy (cryo-EM), atomic force microscopy, and super-resolution microscopy reveal a symmetric trimer with propeller-like blades for the mechanosensitive ion channel PIEZO. However, a conclusive understanding of its conformations in the cell membrane is lacking. Here, we implement a high-vacuum cryogenic shuttle to transfer shock-frozen cell membranes in and out of a cryostat designed for single-particle cryo-light microscopy (spCryo-LM). By localizing fluorescent labels placed at the extremities of the blades of the mouse PIEZO1 protein in unroofed cell membranes, we ascertain three configurations with radii of 6, 12, and 20 nanometers as projected onto the membrane plane. We elaborate on the correspondence of these data with previous reports in the literature. The combination of spCryo-LM with cryo-EM promises to provide quantitative insights into the structure and function of biomolecular complexes in their native environments without the need for chemical fixation or protein isolation, ushering in a new regime of correlative studies in structural biology.*