• Liver and intestine transcript...

*Result*: Liver and intestine transcriptome analysis reveals molecular mechanisms of phytase-driven nutrient utilization and metabolic regulation in hybrid catfish.

Title:
Liver and intestine transcriptome analysis reveals molecular mechanisms of phytase-driven nutrient utilization and metabolic regulation in hybrid catfish.
Authors:
Zhang D; Provincial Key Laboratory for Tropical Hydrobiology and Biotechnology, School of Marine Biology and Fisheries, Collaborative Innovation Center of Marine Science and Technology, School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Haikou 570228/Sanya 572025, China; School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA., Peatman E; School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA., Beck BH; United States Department of Agriculture, Agricultural Research Service, Aquatic Animal Health Research Unit, Auburn, AL 36832, USA., Zhao H; School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA., Mazzola J; School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA., Su B; School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA., Elaswad A; Center of Excellence in Marine Biotechnology, Sultan Qaboos University, Muscat 123, Oman. Electronic address: a.elaswad@squ.edu.om., Ye Z; MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences/Key Laboratory of Tropical Aquatic Germplasm of Hainan Province, Sanya Oceanographic Institution, Ocean University of China, Qingdao 266100/Sanya 572025, China. Electronic address: yezhi@ouc.edu.cn.
Source:
Comparative biochemistry and physiology. Part D, Genomics & proteomics [Comp Biochem Physiol Part D Genomics Proteomics] 2025 Dec; Vol. 56, pp. 101539. Date of Electronic Publication: 2025 May 20.
Publication Type:
Journal Article
Language:
English
Journal Info:
Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101270611 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-0407 (Electronic) Linking ISSN: 1744117X NLM ISO Abbreviation: Comp Biochem Physiol Part D Genomics Proteomics Subsets: MEDLINE
Imprint Name(s):
Original Publication: Amsterdam : Elsevier, 2006-
MeSH Terms:
6-Phytase*/metabolism , 6-Phytase*/pharmacology , Catfishes*/genetics , Catfishes*/metabolism , Catfishes*/growth & development , Liver*/metabolism , Intestinal Mucosa*/metabolism , Fish Proteins*/genetics , Fish Proteins*/metabolism , Transcriptome*, Animal Feed/analysis ; Animals ; Gene Expression Profiling ; Dietary Supplements ; Intestines
Contributed Indexing:
Keywords: Hematological parameters; Hybrid catfish; Phytase; Phytic acid; Transcriptome
Substance Nomenclature:
EC 3.1.3.26 (6-Phytase)
0 (Fish Proteins)
Entry Date(s):
Date Created: 20250525 Date Completed: 20251112 Latest Revision: 20251112
Update Code:
20260130
DOI:
10.1016/j.cbd.2025.101539
PMID:
40413919
Database:
MEDLINE

*Further Information*

*The use of phytase in aquafeeds has gained increasing attention as a strategy to enhance nutritional value and mitigate the adverse effects of phytic acid, especially for diets containing plant-based ingredients. Notwithstanding examples of phytase-induced phenotypic changes, the molecular mechanisms underlying phytase supplementation are not well understood. The present study evaluated the effects of phytase on the transcriptomic profiles in the liver and intestine, as well as on growth, feed conversion ratio (FCR), and hematological parameters of Jubilee × D&B hybrid catfish. Over a 140-day feeding trial, phytase supplementation (2500 phytase units/kg diet) significantly improved growth, FCR, red blood cell count, hematocrit, and total cell count in the blood compared with fish fed the basal diet. By comparing the transcriptomic profiles of phytase-supplemented and control fish, we identified a distinct gene expression profile relative to controls. This profile was characterized by differentially expressed genes (DEGs) associated with mineral metabolism (including iron), energy homeostasis, protein synthesis, carbohydrate and lipid metabolism, and immune response. The putative roles of key DEGs, including their interactions in different metabolic pathways, are discussed. The current study explains the benefits of phytase supplementation on hybrid catfish performance on the molecular level, uncovers the transcriptomic mechanisms controlling these benefits, and provides valuable information for customized functional feeds in aquaculture.
(Copyright © 2025 Elsevier Inc. All rights reserved.)*

*Declaration of competing interest The authors declare that they have no conflict of interest.*