• Suche: "Protein Engineering methods"

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1

Backbone 1H, 15N and 13C resonance assignments of the 27kDa fluorescent protein mCherry
Sette, Marco ; Johnson, Laura Anne ; Jimenez, Ralph ; et al.
Biomol NMR Assign
Sette, M, Johnson, L A, Jimenez, R & Mulder, F A A 2023, 'Backbone 1 H, 15 N and 13 C resonance assignments of the 27kDa fluorescent protein mCherry', Biomolecular NMR Assignments, vol. 17, no. 2, pp. 243-247. https://doi.org/10.1007/s12104-023-10149-z

Photostability Red fluorescent protein Chemical shift Protein engineering mCherry Protein Engineering …
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2

Integrating genetic algorithms and language models for enhanced enzyme design
Nana Teukam, Yves Gaetan ; Zipoli, Federico ; Laino, Teodoro ; et al.
Nana Teukam, Y G, Zipoli, F, Laino, T, Criscuolo, E, Grisoni, F & Manica, M 2025, 'Integrating genetic algorithms and language models for enhanced enzyme design', Briefings in Bioinformatics, vol. 26, no. 1, bbae675. https://doi.org/10.1093/bib/bbae675

biocatalysis computational protein de... enzyme optimization genetic algorithms large language models Algorithms …
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3

Mining and engineering activity in catalytic amyloids
Peña-Díaz, Samuel ; Ferreira, Pedro ; Ramos, Maria João ; et al.
Methods in Enzymology ISBN: 9780443236679
Peña-Díaz, S, Ferreira, P, Ramos, M J & Otzen, D E 2024, 'Mining and engineering activity in catalytic amyloids', Methods in Enzymology, vol. 697, pp. 345-422. https://doi.org/10.1016/bs.mie.2024.03.002

0301 basic medicine Pseudomonas/metabolism Amyloid Amyloid/chemistry tau Proteins Protein Engineering …
Buch
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4

Accelerated Engineering of ELP‐Based Materials through Hybrid Biomimetic‐De Novo Predictive Molecular Design
Timo Laakko ; Antti Korkealaakso ; Burcu Firatligil Yildirir ; et al.
Advanced Materials. 36

computational modeling Models, Molecular α-helical conformation... Molecular protein engineering Elastin/chemistry …
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5

Conjugated Protein Domains as Engineered Scaffold Proteins
Lenne J. M. Lemmens ; Christian Ottmann ; Luc Brunsveld
Bioconjug Chem

0301 basic medicine 0303 health sciences Proteins Hydrogels Hydrogels/chemistry Protein Engineering …
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6

Use of the chicken lysozyme 5' matrix attachment region to generate high producer CHO cell lines.
Girod, P.A. ; Zahn-Zabal, M. ; Mermod, N.

Animals CHO Cells/metabolism Chickens Cricetinae Cricetulus Genetic Enhancement/meth... …
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7

Effect on antibody and T-cell responses of mixing five GMP-produced DNA plasmids and administration with plasmid expressing GM-CSF
Sedegah, M. ; Charoenvit, Y. ; Aguiar, J. ; et al.

Animals Antibodies Protozoan/*biosynthesis... Inbred BALB C Mice Inbred C57BL Plasmids/*a... DNA/*administration & do...
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8

siRNA-based inhibition specific for mutant SOD1 with single nucleotide alternation in familial ALS, compared with ribozyme and DNA enzyme
Yokota, T. ; Miyagishi, M. ; Hino, T. ; et al.

Amyotrophic Lateral Scle... Catalytic/genetics/*meta... Enzymologic/genetics Gen... Site-Directed Neuroblast... Catalytic/genetics/*meta... Small Interfering/geneti...
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9

Development of stable cell lines for production or regulated expression using matrix attachment regions.
Zahn-Zabal, M. ; Kobr, M. ; Girod, P.A. ; et al.

Animals CHO Cells Cell Line Chickens Chromatin/genetics Cricetinae …
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10

A new method for post-translationally labeling proteins in live cells for fluorescence imaging and tracking
Hinrichsen, M. ; Lenz, M. ; Edwards, J. M. ; et al.
Hinrichsen, M, Lenz, M, Edwards, J M, Miller, O K, Mochrie, S G J, Swain, P S, Schwarz-Linek, U & Regan, L 2017, ' A new method for post-translationally labeling proteins in live cells for fluorescence imaging and tracking ', Protein engineering, design & selection : PEDS, vol. 30, no. 12, pp. 771-780 . https://doi.org/10.1093/protein/gzx059

0301 basic medicine Post-translational label... Fluorescent Dyes/analysi... QH301 Biology NDAS Cell Tracking/methods …
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11

How Does Replacement of the Axial Histidine Ligand in Cytochrome c Peroxidase by Nδ-Methyl Histidine Affect Its Properties and Functions? A Computational Study
Lee, Calvin W. Z. ; Bin Mubarak, Muhammad Qadri Effendy ; Green, Anthony ; et al.
Lee, C W Z, Bin Mubarak, M Q E, Green, A & De Visser, S 2020, 'How Does Replacement of the Axial Histidine Ligand in Cytochrome c Peroxidase by Nδ-Methyl Histidine Affect Its Properties and Functions? A Computational Study', International Journal of Molecular Sciences, vol. 21, no. 19, 7133, pp. 1-18. https://doi.org/10.3390/ijms21197133

Density functional theor... Enzyme engineering Enzyme models Epoxidation Heme enzymes Hydroxylation …
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12

Towards further reduction and replacement of animal bioassays in prion research by cell and protein misfolding cyclic amplification assays
Boerner, Susann ; Wagenführ, Katja ; Daus, Martin L. ; et al.
Laboratory Animals. 47:106-115

PrPSc Proteins/genetics 0301 basic medicine PrPSc Proteins/chemistry Protein Folding Neuroglia/metabolism PrPSc Proteins …
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13

Bone formation of block and particulated biphasic calcium phosphate lyophilized with Escherichia coli–derived recombinant human bone morphogenetic protein 2 in rat calvarial defects
Jin-Woo, Kim ; Kyung-Hee, Choi ; Jeong-Ho, Yun ; et al.
Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology. 112:298-306

Male 0301 basic medicine Skull/surgery Bone Regeneration Bone Regeneration/physio... Bone Morphogenetic Prote... …
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14

Tailoring protein nanomechanics with chemical reactivity
Beedle, Amy ; Mora, Marc ; Lynham, Steven ; et al.
Nat Commun
Nature Communications, Vol 8, Iss 1, Pp 1-11 (2017)
Beedle, A E M, Mora, M, Lynham, S, Stirnemann, G & Garcia-Manyes, S 2017, ' Tailoring protein nanomechanics with chemical reactivity ', Nature Communications, vol. 8, no. 1, 15658, pp. 1-11 . https://doi.org/10.1038/ncomms15658

Models, Molecular 0301 basic medicine Protein Folding Cysteine/chemistry Oxygen/chemistry Protein Conformation …
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15

Engineering Antibody Heavy Chain CDR3 to Create a Phage Display Fab Library Rich in Antibodies That Bind Charged Carbohydrates
Schoonbroodt, Sonia ; Steukers, Mieke ; Viswanathan, Malini ; et al.
The Journal of Immunology. 181:6213-6221

0301 basic medicine Immunology Molecular Sequence Data Static Electricity Bacteriophage M13/immuno... Oligosaccharides …
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16

Challenges Associated with Heterologous Expression of Flavobacterium psychrophilum Proteins in Escherichia coli
Shah, Devendra H ; Cain, Kenneth D ; Wiens, Gregory D ; et al.
Marine Biotechnology. 10:719-730

0301 basic medicine Escherichia coli - metab... Inclusion Bodies - metab... Flavobacterium - genetic... Protein Engineering Flavobacterium …
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17

Directed evolution on the cold adapted properties of TAB5 alkaline phosphatase
Koutsioulis, D. ; Wang, E. ; Tzanodaskalaki, M. ; et al.
Protein Engineering Design and Selection. 21:319-327

Recombination, Genetic 0301 basic medicine Protein Denaturation Protein Folding 0303 health sciences Binding Sites …
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18

Structure-based protein engineering efforts with a monomeric TIM variant: the importance of a single point mutation for generating an active site with suitable binding properties: The importance of a single point mutation for generating an active site with suitable binding properties
Alahuhta, Markus ; Salin, Mikko ; Casteleijn, Marco G. ; et al.
Protein engineering design and selection 21, 257-266 (2008). doi:10.1093/protein/gzn002
Protein engineering design and selection

Models, Molecular 0301 basic medicine Acetone: analogs & deriv... Mutant Proteins: metabol... Protein Conformation Glutamic Acid …
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19

Enhancing the stability and solubility of TEV protease using in silico design
Cabrita, Lisa D. ; Gilis, Dimitri ; Robertson, Amy L ; et al.
Protein Science. 16:2360-2367

Models, Molecular 0301 basic medicine Endopeptidases -- chemis... Protein Denaturation Guanidine -- pharmacolog... Molecular Conformation …
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20

Computational Protein Design Promises to Revolutionize Protein Engineering
Alvizo, Oscar ; Allen, Benjamin D. ; Mayo, Stephen L.
BioTechniques, Vol 42, Iss 1, Pp 31-39 (2007)

Models, Molecular 0301 basic medicine 0303 health sciences QH301-705.5 Protein Conformation Molecular …
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